秋冬季婴幼儿轮状病毒感染分子流行病学调查  

Molecular epidemiologic distribution of rotavirus infection in autumn and winter among infants in Zhaotong area of Yunnan province,2010

作　　者：赵晓南[1] 李鸿钧[1] 吴晋元[1] 张光明[1] 易山[1] 杨星[1] 郜岩[2] 贾琴妹[1] 木国法 孙茂盛[1] 

机构地区：[1]北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室,云南昆明650118 [2]昆明医学院 [3]云南省昭通市第一人民医院

出　　处：《中国公共卫生》2012年第6期823-825,共3页Chinese Journal of Public Health

基　　金：云南省社会发展科技计划国际合作项目(2008CA028);云南省应用基础研究计划面上项目(2009CD142)

摘　　要：目的监测2010年云南昭通地区秋冬季腹泻患儿感染轮状病毒的流行趋势,了解流行毒株的基因型特征。方法 2010年9—12月在云南省昭通市第一人民医院采集94份就诊的腹泻患儿粪便,应用胶体金法、逆转录聚合酶链式反应及PAGE电泳等方法,对样品进行轮状病毒抗原检测,其中8份阳性样品进行VP7基因测序分析及基因型分型。结果腹泻病患排泄物中轮状病毒抗原阳性比例为54.3%(51/94);8份轮状病毒基因序列分析,其中7份为G1型,1份为G3型;核苷酸同源性分析发现检测样品中轮状病毒主要与在中国辽宁、印度和泰国发现的流行株相似。结论 2010年昭通地区秋冬季患儿腹泻主要由轮状病毒引起,其中以G1型为主,其次为G3型。Objective To investigate etiology,epidemiologic and molecular virology features of rotavirus causing infant diarrhea in Zhaotong region in autumn and winter of 2010.Methods A total of 94 pieces of stool specimen collected from infants with acute diarrhea in the First People's Hospital of Zhaotong City from September to December,2010 were analyzed with colloidal gold test,reverse transcription-PCR,and polyacylamide gel electrophoresis and then 8 stool specimens were selected from the 94 specimens and analyzed by VP7 gene sequencing and genotyping.Results Rotaviruses were detected in 54.3% of samples.Rotavirus genome sequence analyses showed 7 samples were G1 and one sample was G3.Phylogenetic analysis based on nucleotide sequence revealed that the rotavirus isolated in Liaoning province,India and Thailand were very similar strains.Conclusion The infant diarrhea epidemic in the autumn and winter of 2010 in Zhaotong area was mainly caused by G1 and G3 genotype rotavirus.

关 键 词：轮状病毒 婴幼儿 腹泻 基因型 

分 类 号：R512.5[医药卫生—内科学]