动脉粥样硬化患者基质金属蛋白酶-9及其抑制物-1基因表达与痰浊证素的相关性研究  

Study of Correlation Relationship in MMP-9,TIMP-1 and Phlegm Syndrome in Atherosclerosis Patients

作　　者：谈晓东[1,2] 苏伟[1,2] 高枫[1,2] 周春刚[1,2] 陆曙[1,2] 

机构地区：[1]南京中医药大学无锡附属医院 [2]无锡市中医医院,江苏无锡214001

出　　处：《中国中医急症》2012年第6期868-869,886,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：江苏省自然科学基金资助项目(BK2008117)

摘　　要：目的分析动脉粥样硬化(AS)患者基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶抑制物-1(TIMP-1)的基因表达水平与痰浊证素的相关性,探讨MMP-9、TIMP-1基因表达水平作为AS患者中医"痰浊证素"客观指标的可行性。方法将AS患者分为痰浊证素组30例,非痰浊证素组48例,通过实时荧光定量PCR(RT-PCR)技术进行MMP-9和TIMP-1mRNA表达水平的定量检测。结果痰浊证素组MMP-9和TIMP-1基因表达水平均明显高于非痰浊证素组,差异具有统计学意义。结论 MMP-9及TIMP-1基因表达可作为中医痰浊证素的客观指标。Objective： To analysis the relationship between phlegm syndrome element and indices such as matrix metalloproteinase-9 （MMP-9） and matrix metalioproteinase inhibitor-1 （TIMP-1） gene expression and to study that the feasibility of the MMP-9 and TIMP-1 gene expressions could be used as the objective indicators for phlegm syndrome element in patients with AS. Methods： 30 cases with phlegm syndrome and 48 cases with non- phlegm syndrome were chosen in the study. The levels of mRNA expressions including MMP-9 and TIMP-1 were detected by real-time fluorescence quantitative PCR （RT-PCR） technology. Results： The levels of MMP-9 and TIMP-1 gene expression in the subgroup of phlegm syndrome element were significantly higher than those in the non-phlegm syndrome. Conclusion： MMP-9 and TIMP-1 gene expressions can be the objective of phlegm syn- drome element prime targets.

关 键 词：基质金属蛋白酶-9 基质金属蛋白酶抑制物-1 动脉粥样硬化 痰浊证素 

分 类 号：R543.5[医药卫生—心血管疾病]