褐藻多糖硫酸酯对阿霉素肾硬化大鼠肾组织FN、TGF-β、MMP-9的影响  

Effect of Fucoidan on FN,TGF-β and MMP-9 in Adriamycin-induced Glomerulosclerosis Rats

作　　者：陈雪风[1] 吴汉利[1] 

机构地区：[1]山东省潍坊市益都中心医院,山东青州262500

出　　处：《中国中医急症》2012年第6期926-928,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：山东省潍坊市科学技术局科研课题(201104094)

摘　　要：目的观察褐藻多糖硫酸酯(FPS)对阿霉素肾硬化大鼠肾脏的保护作用。方法将40只SD大鼠分成正常组、模型组、海昆肾喜组(褐藻多糖硫酸酯组)、盐酸贝那普利组。建立阿霉素肾硬化大鼠模型,分别采用相应药物干预;8周后观察各组大鼠肾脏病理改变,PAS观察肾小球系膜基质,免疫组化观察肾脏组织纤连蛋白(FN),Western blot观察转化生长因子β(TGF-β)和金属蛋白酶-9(MMP-9)的表达。结果 FPS能减轻肾小球系膜基质增生,且能减少TGF-β、FN表达,上调肾皮质MMP-9的表达。相关性分析研究显示,TGF-β值与FN、系膜基质指数呈正相关;MMP-9值与FN、系膜基质指数呈负相关。结论 FPS可能通过调控TGF-β及MMP-9表达,减轻FN沉积,从而抑制肾小球硬化。Objective：To investigate the effects and mechanism of fucoidan on adriamycin-induced glomeru- loscterosis in rats. Methods：40 rats were randomly divided into the normal group,the model group,the fucoidan group and the benazepril hydrochloride groups. After 8 weeks,renal pathology and immunohistochemistry were assayed to measure the expression of fibronectin （FN）. Western blot was used to examine the transformor growth factor β （TGF-β） protein and matrix metalloproteinases 9 （MMP-9） protein. Results：The expression of glomernlar mesangial matrix of adrianmyein-indueed,FN ,TGF-β were decreased by fucoidan. The expression of MMP-9 was increased by fucoidan. Correlation studies have shown that protein of TGF-β and glomerular mesangial matrix, FN were positively correlated. Meanwhile,the protein of MMP-9 and glomernlar mesangial matrix, FN were negatively correlated. Conelusion：Fucoidan plays an important role on prevention and treatment for glomerulosclerosis through the regulation of inflammatory factors and the inhibition of FN.

关 键 词：褐藻多糖硫酸脂 肾小球硬化症 FN TGF-β1 MMP-9 

分 类 号：R285.5[医药卫生—中药学]