转AtDREB2A基因苜蓿的耐碱性分析  

Alkaline Tolerance Analysis of Transgenic Alfalfa with AtDREB2A Gene

作　　者：文益东[1] 才华[2] 柏锡[2] 纪巍[2] 王臻昱[2] 魏正巍[2] 朱延明[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《作物杂志》2012年第3期32-35,共4页Crops

基　　金：国家自然科学基金(31171578);黑龙江省高校科技创新团队建设计划(2011TD005);黑龙江省重大科技攻关项目(GA06B103);东北农业大学创新团队项目(CXT004-1)

摘　　要：对已获得的转基因苜蓿进行了RT-PCR检测,确定AtDREB2A基因已在转基因苜蓿中超量表达。并研究了转AtDREB2A基因苜蓿在碱胁迫(100mmol/L,pH8.5)下表型和生理生化指标的变化。结果表明,转AtDREB2A基因苜蓿的相对质膜透性及丙二醛含量显著低于野生型株系,株高、叶绿素及根系活力明显高于野生型株系,说明AtDREB2A基因的超量表达提高了转基因苜蓿的耐碱能力。AtDREB2A overexpression was confirmed by RT-PCR.The AtDREB2A-overexpression alfalfa plants were treated with NaHCO3（100mmol/L,pH8.5） and the change of physiological indexes were measured.The results showed that relative membrane permeability and MDA content of transgenic alfalfa were less than those in wild type.Moreover,the shoot length,chlorophyll content and root activity of transgenic alfalfa were significantly higher than those in wild type.The results indicated that overexpression of the AtDREB2A gene conferred enhanced alkaline tolerance to the transformed alfalfa.

关 键 词：紫花苜蓿 AtDREB2A 转基因 耐碱性 

分 类 号：S541.9[农业科学—作物学]