甜瓜种质鉴定的高效引物(引物组合)适用性分析  

Suitability Analysis on Highly Active Primers(Primer Combinations)of Germplasm Identification in Cucumis melo L.

作　　者：王掌军[1] 王建设[2] 刘生祥[1] 蒋全熊[1] 张晓岗[1] 马宏玮[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]国家蔬菜工程技术研究中心,北京100089

出　　处：《作物杂志》2012年第3期136-140,共5页Crops

基　　金：宁夏大学科学研究基金重点项目(NDZR10-9);宁夏大学农学院青年教师科研项目

摘　　要：以30份甜瓜种质资源为材料,采用RAPD(random amplified polymorphic DNA)、DAMD(directed amplification of minisatellite-region DNA)和SRAP(sequence-related amplified polymorphism)3种分子标记构建各自的DNA指纹图谱。针对图谱上扩增的条带分子量大小和分布,用统计软件逐一聚类,根据不同引物(引物组合)所得聚类图鉴别的品种数目,筛选出高分辨率的4个RAPD引物、3个DAMD引物和6个SRAP引物组合。同时,利用高效引物(引物组合)评价了甜瓜遗传多样性,将30份甜瓜分为薄皮和厚皮两类,厚皮甜瓜包括网纹和无网纹两种类型。The study constructed each DNA fingerprint map by RAPD,DAMD and SRAP molecular markers in 30 melon germplasms.Statistical software was used to cluster one by one in collection with the dimension and distribution of the molecular weight in the map amplified.Based on the cultivar numbers identified by the clustering map from different primers（primer combinations）,four RAPD primers,three DAMD primers and six SRAP primer combinations with highly distinguished rate were screened.At the same time,the genetic diversity of melons were evaluated by these high active primers（primer combinations）,30 melons were divided to two kinds,ie,C.melo sp.conomom and C.melo sp.melo,C.melo sp.melo included C.melo cantalupo and C.melo reticulatus.

关 键 词：甜瓜 种质鉴定 高效引物(引物组合) 

分 类 号：S652[农业科学—果树学]