脉络宁对原代大鼠海马神经元在氧糖剥夺/复氧中的保护作用  

Neuroprotective effect of Mailuoning on injury after oxygen glucose deprivation/reoxygenation in primary cultures of rat hippocampal neurons

作　　者：荣国铃[1] 吴世政[2] 

机构地区：[1]四川省达州市中心医院,达州635000 [2]青海省人民医院神经内科,西宁810007

出　　处：《中国新药杂志》2012年第11期1278-1282,共5页Chinese Journal of New Drugs

摘　　要：目的:观察脉络宁对氧糖剥夺/复氧(oxygen glucose deprivation/reoxygenation,OGD/R)诱导的SD大鼠原代培养海马神经元损伤的保护作用及可能机制。方法:原代培养从新生24 h内SD乳鼠取材的海马神经元,建立氧糖剥夺30 min/复氧8 h海马神经元损伤模型。实验分为正常对照组、OGD/R模型组、脉络宁低、中、高剂量组(5,10和20μg.mL-1)。采用Hoechest33258染色测定细胞凋亡,免疫细胞化学检测各组细胞中脑红蛋白(neuroglobin,NGB)及Bcl-2的表达,PCR检测其mRNA的表达情况。结果:与OGD/R模型组比较,各剂量脉络宁组神经元凋亡率均不同程度降低(P<0.01),且随药物浓度的升高而降低;NGB和Bcl-2蛋白表达不同程度地升高(P<0.01),且随药物浓度升高而升高;NGB及Bcl-2蛋白的mRNA亦升高(P<0.01)。结论:脉络宁发挥神经保护作用可能的机制之一是抑制OGD/R诱导的海马神经元细胞凋亡,促进NGB和Bcl-2蛋白的表达。Objective： To determine the neuroprotective effect of Mailuoning against oxygen glucose deprivation/reoxygenation（OGD/R）-induced injury and the possible mechanisms.Methods： Primarily cultured hippocampal neurons from newborn SD rats（24 h） were subjected to 30-min OGD/8-h R.The neurons were treated with Mailuoning 5,10,20 μg·mL^-1.Apoptotic cells were analyzed by Hoechest33258 staining.Neuroglobin（NGB）and Bcl-2 expression were analyzed by immunocytochemistry.The mRNA expression of NGB and Bcl-2 was analyzed by RT-PCR.Results： Compared with OGD/R control,Mailuoning at 3 concentrations significantly decreased neuronal apoptosis（P〈0.01） concentration-dependently,and increased the expression of NGB protein with the increasing of drug concentration（P〈0.01）.It also significantly increased the mRNA expression of NGB and Bcl-2（P〈0.01）.Conclusion： Mailuoning has a neuroprotective effect on OGD/R injury in hippocampal neurons,which is related to inhibiting apoptosis,and increasing NGB and Bcl-2 expression.

关 键 词：脉络宁 海马神经元 氧糖剥夺/复氧 脑红蛋白(NGB) Bcl-2 mRNA 

分 类 号：R971[医药卫生—药品]