用体视学方法测定兔眼钝挫伤后脉络膜形态学改变  

Determination of morphological changes in choroid of rabbits' eyes after contusion by stereological method

作　　者：吴燕[1] 林立[1] 蒋炜[1] 

机构地区：[1]成都军区总医院眼科,成都610083

出　　处：《西南国防医药》2012年第6期602-604,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的运用体视学方法研究兔眼钝挫伤后脉络膜形态学指标的变化,了解兔眼血循环的改变。方法以自由落体击伤试验兔右眼,损伤能量为3.3 J,造成灰兔单眼轻度钝挫伤模型,左眼正常对照。将兔眼眼球进行HE染色观察脉络膜病理学改变,并采用生物体视学中卡瓦列里原理法测定脉络膜的体积(Vc)、脉络膜血管的体积密度(Vv)、脉络膜血管的体积(Vb)。结果伤后脉络膜血管体积、血管的体积密度以及脉络膜血管的体积均较正常眼球相比明显减小。伤眼脉络膜体积为正常对照组的87.04%(P<0.05);伤眼脉络膜血管体积密度为正常眼球的87.70%(P<0.05);伤眼脉络膜血管体积为正常对照组值的69.94%(P<0.01)。结论生物体视学中卡瓦列里原理法测定兔眼脉络膜体积和脉络膜血管体积有效可靠,并能量化比较损伤前后脉络膜及其血管形态学的变化。眼球钝挫伤后脉络膜受到明显损害,脉络膜血管体积明显减小,可能导致脉络膜循环血量减少,从而影响钝挫伤后视网膜功能的恢复。Objective To investigate the morphological index changes in the choroid of hares＇ eyes after contusion by the method of stereology, and to understand the changes in the blood circulation of rabbits＇ eyes. Methods The right eyes of the rabbits were injured by the free - falling blunt body with the damage energy of 3.3 J. The models of light contusion in the right eyes were established, and their left eyes were used as the normal control. HE staining was made in the right eyeballs in order to observe the pathological changes of choroid. The volume （ Vc ）, blood vessel volume density （Vv） and blood vessel volume （ Vb ） of choroid were detected based on the Cavalieri principle in biological stereology. Results After the injury, compared with the Vc, Vv and Vb of the normal eyeballs ,those of the injured eyes decreased significantly. The Vc of the injured eyes accounted for 87.04% of that of the normal eyeball（ P 〈 0.05 ） ;the Vv accounted for 87.70% （ P 〈 0.05 ） ; the Vb accounted for 69.94% （ P 〈 0.01 ）. Conclusion The Vc and Vb detected by the Cavalieri principle in biological stereology are effective and reliable, which can be used to make quantitative comparison of morphological changes of choroid and the blood vessel before and after the injury. After the contusion of eyeball, the choreid is obviously damaged, and the Vb significantly diminishes, which may results in the decrease of blood circulation volume in the choreid and further affect the recovery of retina function.

关 键 词：眼球钝挫伤 脉络膜损伤 体视学 兔 

分 类 号：R779.1[医药卫生—眼科]