转cry1Ac+cry2Ab基因棉与转cry1Ac+EPSPS基因棉荒地的生存竞争能力  

Studies on the competitive ability of transgenic cry1Ac+cry2Ab cotton and transgenic cry1Ac+EPSPS cotton in wasteland

作　　者：张兴华[1] 田绍仁[1] 张天玉[1] 李捷[1] 乔艳艳[1] 

机构地区：[1]江西省棉花研究所,江西九江332105

出　　处：《生物安全学报》2012年第2期119-124,共6页Journal of biosafety

基　　金：国家"十二五"转基因生物新品种培育重大专项(2011ZX08011-002)

摘　　要：【背景】此研究为"十二五"转基因生物新品种培育国家项目中创建新的转基因棉花品种环境安全评价技术而设。【方法】以转双价双Bt抗虫基因(cry1Ac+cry2Ab)棉和转双价抗虫、抗除草剂基因(cry1Ac+EPSPS)棉为观察品种,非转基因棉赣棉11号为对照品种,在荒地用撒播和3cm深度播种2种方式,于2011年5月～2012年3月对棉花出苗率、株高、生育进程、棉吐絮瓣数、絮瓣脱落率、自生苗等生存竞争能力进行比较,检测、评价其杂草化的风险,并探讨、验证检测技术的可行性。【结果】在荒地条件下,以2种方式播种的转cry1Ac+cry2Ab基因棉和转cry1Ac+EPSPS基因棉与非转基因棉相比,上述各项指标的竞争能力总体上未表现显著优势。【结论与意义】转cry1Ac+cry2Ab基因棉和转cry1Ac+EPSPS基因棉在荒地条件下生长无杂草化风险。同时,研究证明,在荒地自然生态条件下,可以采用撒播和3cm深度播种方法检测新的转基因棉花品种在生存竞争能力上的杂草化风险,在测评上有互为参照效应,为定性评价新的转基因棉花品种的杂草化风险提供了保障。[ Background] This study is to create a new environmental safety assessment technology of transgenic cotton in the ＂Twelfth Five＂ national project, which is aim at cultivating new variety of genetically modified organism. [ Method] Three experiment materials were put in trial, including two transgenic cottons with two Bt genes （crylAc, cry2Ab） and the double insect and herbi- cide-resistant genes respectively, which were the observed species, and one non-transgenic cotton Ganmian No. 11, which was the check test variety. The three materials were seeded on the earth＇s surface and under the three centimeters depth of the earth on May 5, 2011 in order to compare their seeding emergence rate, plant height, reproductive process, the number of cotton boll opening valve, the floc valve dropping rate and survival competitiveness of the autogenetic seedling rate, evaluate their risk of weediness, discuss and verify the feasibility of this detection technology. [ Result] The differences of all above survival competitive ability be- tween the double price of Bt gene （crylAc, cry2Ab） cotton, the double price of insect and herbicide-resistant gene cotton and the control variety were less than 5% in general, which indicated the two transgenic cotton had no weediness risk. [ Conclusion and sig- nificance] It was not only feasible to use seeding on the earth＇s surface as well as seeding under the three centimeters depth of the earth as detecting whether the survival competitive ability of new transgenic cotton has risk of weediness in the wasteland, but also had interactive reference effect on evaluation, which provided more reliable insurance on evaluation and had the significance of tech- nique location.

关 键 词：双价双Bt抗虫基因棉 cry1Ac+cry2Ab基因 双价抗虫 抗除草剂基因棉 5-烯醇丙酮酰莽草酸-3-磷酸合酶基因 生存竞争能力 杂草化风险 

分 类 号：S562[农业科学—作物学]