CYP11B2基因多态性与慢性心力衰竭及醛固酮水平的相关性研究  

Relationship of CYP11B2 gene polymorphism with chronic heart failure and plasma aldosterone level

作　　者：朱清艳[1] 顾水明[2] 

机构地区：[1]江苏大学临床医学院,江苏镇江212001 [2]上海市徐汇区中心医院心内科,上海200031

出　　处：《江苏大学学报（医学版）》2012年第3期262-264,共3页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:研究醛固酮合酶(CYP11B2)基因-344C/T多态性与慢性心力衰竭(chronic heart failure,CHF)及血浆醛固酮水平的关系。方法:采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测96例CHF患者和160例健康对照的CYP11B2基因-344C/T多态性,应用ELISA方法检测血浆醛固酮水平,分析CYP11B2基因-344C/T多态性与CHF及醛固酮水平的关系。结果:CHF组和对照组基因型和等位基因频率比较差异均无统计学意义(χ2=2.50,P=0.287;χ2=2.31,P=0.129),CHF组TT、CT、CC三组基因型血浆醛固酮水平分别为(181.49±46.23)、(160.31±28.82)、(148.02±24.39)pg/ml,组间比较差异有统计学意义(TT与CC比较,t=1.99,P=0.029;TT+CT与CC比较,t=1.71,P=0.043)。结论:CYP11B2基因-344C/T多态性与慢性心力衰竭的发生不相关,CHF患者血浆醛固酮水平与CYP11B2基因-344C/T多态性相关。Objective： To study the relationship between CYP11B2 gene-344T/C polymorphism,chronic heart failure（CHF） and plasma aldosterone levels in CHF patients.Methods： CYP11B2 gene-344T/C polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） in 256 subjects（96 patients with CHF and 160 controls）,and the levels of plasma aldosterone in patients with CHF was assessed by ELISA.Results： No significant difference was found in genotype or allele frequency distribution between groups of control and CHF（χ^2=2.50,P=0.287;χ^2=2.31,P=0.129）.The levels of plasma aldosterone in patients with CHF was（181.49±46.23）pg/ml in TT genotype class,（160.31±28.82）pg/ml in TC class and（148.02±24.39）pg/ml in CC class;TT vs.CC,t=1.99,P=0.029;TT ＋ CT vs.CC,t=1.71,P=0.043.Conclusion： There was no association between the-344T/C polymorphism of CYP11B2 gene and CHF.The-344T/C polymorphism of CYP11B2 gene was associated with plasma aldosterone levels in patients with CHF.

关 键 词：慢性心力衰竭 醛固酮合酶(CYP11B2)基因多态性 醛固酮 

分 类 号：R541.6[医药卫生—心血管疾病]