大鼠肠内菌转化连翘苷的代谢产物  

Metabolites of Phillyrin by Rat Intestinal Flora

作　　者：刘西哲[1] 生宁[1] 霍好利[1] 孟岩[2] 张兰桐[1] 

机构地区：[1]河北医科大学药学院药物分析教研室,河北石家庄050017 [2]石家庄市中心医院药剂科,河北石家庄050011

出　　处：《中国医药工业杂志》2012年第6期455-458,共4页Chinese Journal of Pharmaceuticals

基　　金：河北省科技支撑计划项目(09276423D);石家庄市科技局指令计划指导项目(10146513A)

摘　　要：应用液相色谱-串联质谱法研究肠内菌转化连翘苷的代谢产物。将连翘苷与大鼠肠内菌于体外厌氧温孵培养,在温孵液中检测到连翘苷及其3种代谢产物。放大制备了转化产率高的代谢物,依据1H NMR和ESI-MS数据推测代谢物结构并假设了连翘苷可能的代谢途径。Phillyrin and its metabolites in rat intestinal flora were identified by LC-MS/MS. Phillyrin was anaerobically incubated with rat intestinal flora in vitro. The parent drug and three metabolites were found in theincubation solution. The metabolites with high conversion yields were prepared by preparative liquid chromatography. The chemical structures of three metabolites were identified by 1H NMR and ESI-MS, which inferred possible microbialtransformation pathways of phillyrin by rat intestinal flora.

关 键 词：连翘苷 肠内菌 代谢 核磁共振 液相色谱-串联质谱 结构解析 

分 类 号：TQ460.72[医药卫生—药物分析学]