非接触调频模式AFM的发展及生物学应用  

A review on non-contact frequency-modulation AFM and its application in biology

作　　者：李林[1] 张金金[1] 胡钧[1] 

机构地区：[1]中国科学院上海应用物理研究所,上海201800

出　　处：《核技术》2012年第6期462-466,共5页Nuclear Techniques

基　　金：国家重点基础研究发展计划(973项目:2007CB936000)

摘　　要：非接触调频模式原子力显微镜(AFM)可实现高分辨成像,其探针针尖与样品间的相互作用力非常小,能最大限度减少样品形变。本文综述非接触调频模式原子力显微镜在真空和溶液中的高分辨成像发展,并以DPPC磷脂双分子层和人类免疫球蛋白IgM为例,介绍了该系统在生物结构学研究中的应用。Performance and imaging mode of atomic force microscope （AFM） have been in continuous development and now AFMs are operated in contact mode, tapping mode, or non-contact frequency-modulation mode. A non-contact frequency-modulation AFM achieves high resolution, and the sample distortion is greatly reduced because of the very small interaction force between the tip and sample. In this paper, we give a review on high resolution imaging of non-contact frequency-modulation AFM in vacuum and in physiological condition for better application in biology. Applications of the system in structural biology using the DPPC bilayer and IgM are given as samples.

关 键 词：原子力显微镜 非接触 高分辨 调频模式 

分 类 号：Q334[生物学—遗传学]