温针灸对吗啡戒断大鼠NR2B受体表达影响的研究  

Acupuncture with Warm Needle on the Expression of NR2B Receptor of Rats after Morphine Withdrawal

作　　者：卫哲[1] 董哲 孙远征[1] 尚颖[1] 

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [2]哈尔滨市儿童医院,黑龙江哈尔滨150010

出　　处：《针灸临床杂志》2012年第5期56-58,共3页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江省青年科学基金项目;编号:QC2011C040;黑龙江中医药大学校基金项目;编号:201013

摘　　要：目的:观察温针灸对吗啡戒断大鼠空间学习能力及脑区NR2B受体表达的影响。方法:采用逐日背部皮下注射盐酸吗啡注射液,末次注射后3 h给予纳络酮快速戒断,建立吗啡戒断大鼠模型。给予温针灸治疗后,采用Morris水迷宫测试大鼠空间学习能力,免疫组化方法检测伏隔核脑区NR2B受体的表达。结果:温针灸组大鼠平台逃避潜伏期明显缩短(P<0.01)。温针灸组大鼠NAc脑区NMDA-NR2B受体表达增高(P<0.05)。结论:温针灸能提高大鼠的空间学习能力,其机制可能与调整脑区NR2B受体表达有关。Objective：To observe rats’ interpace learning ability and the expression of NR2B receptor in brain area by acupuncture with warm needle after morphine withdrawal.Methods：Establish a morphine dependent SD rat model.We adopted hypodermic injection by morphine in back day by day,and injected naloxone in order to rapid withdrawal after the last injection of three hours.We used Morris water maze to testing rats’ interpace learning ability.The expression of NR2B receptor in NAc brain areas of rats were measured by immunohistochemical method.Results：The escape latent period of flat roof in acupuncture with warm needle group rats was abbreviated obviously（P〈0.01）.The expression of NMDA-NR2B receptor heightened acupuncture with warm needle group（P〈0.05）.Conclusion：Acupuncture with warm needle can improve the ability of interpace learning.The mechanism is related to adjusting the expression of NR2B receptor probably.

关 键 词：温针灸 吗啡 戒断 MORRIS水迷宫 NR2B受体 

分 类 号：R245.315[医药卫生—针灸推拿学]