HPLC法测定茯苓中茯苓酸的含量  

Determination of Contents of Pachymic Acid in Poria by HPLC

作　　者：方东军[1] 赵润琴[1] 张晓娟[2] 

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2012年第3期54-56,共3页Information on Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金项目(D200928)

摘　　要：目的:建立测定茯苓药材中茯苓酸含量的反相高效液相色谱法。方法:采用HPLC法。色谱柱为Diamonsil C18柱(250mm×4.6mm,5μm),流动相为甲醇-水溶液梯度洗脱,流速为1.0mL/min,柱温为30℃,检测波长为250nm。结果:茯苓酸进样浓度在50～3 000μg/mL之间有良好的线性关系。平均加样回收率为98.53%,RSD(n=6)为2.24%。结论:HPLC法可作为茯苓质量控制的一个有效方法。Objective：To develop HPLC for the determination of pachymic acid in Poria（the dry sclerotium of Poria cocos（schw.）Wolf.）.Methods：The separation was performed on a Diamonsil C18 column（250mm×4.6mm,5μm） with methanol-water as themobile phase at a flow rate of 1.0mL/min.The detective wavelength was set at 250nm and the column temperature was 30℃.Results：There were good linear relationships between the concentrations and the peak areas of pachymic acid in the range of 50~3000μg/mL（R2=0.999 5）.The recoveries were found to be 98.53% with RSD of 2.24%（n=6）.Conclusion： This meothod is suitable for the quality control of Poria.

关 键 词：反相高效液相色谱法 茯苓酸 茯苓 

分 类 号：R284[医药卫生—中药学]