甲氧西林耐药溶血葡萄球菌的耐药基因检测及同源性分析  

Detection of drug-resistant related genes and analysis of homology in methicillin-resistant Staphylococci haemolyticus

作　　者：叶海油 林镯[2] 庄建华 阳韬[2] 陆小蒟[2] 

机构地区：[1]温州市鹿城区人民医院,325007 [2]温州医学院附属第一医院

出　　处：《浙江医学》2012年第10期776-779,共4页Zhejiang Medical Journal

基　　金：温州市鹿城区科技计划项目（S090210）

摘　　要：目的 了解本地区甲氧西林耐药溶血葡萄球菌（MRSH）携带耐药基因情况及其流行状况,提供临床治疗及感染控制的依据.方法 耐药基因的检测采用PCR,菌株同源性分型采用随机扩增DNA 多态性（RAPD）技术.结果 65 株MRSH 的mecA、Aac（6＇）/aph（2＇＇）、aph（3＇）-Ⅲ、ermB、ermC 和tetM 耐药基因的阳性率分别为100.0%、93.8%、76.9%、7.7%、96.9%和4.6%,未检测出ermA、tetO 和vanA 耐药基因.依照RAPD 检测方法将65 株MRSH 分为3型,其中A 型占83.1%.结论 本地区的MRSH 携带高比例的mecA、Aac（6＇）/aph（2＇＇）、aph（3＇）- Ⅲ和ermC 耐药基因,RAPD分析MRSH 的流行株以A 型为主.Objective To investigate the prevalent strains in methicillin-resistant Staphylococci haemolyticus （MRSH） and drug-resistant related genes in MRSH in the region. Methods Drug-resistant related genes were detected by polymerase chain reaction （PCR） and homologous strains were typed by random amplified polymorphic DNA （RAPD）. Results The test of resis- tant-related genes in MRSH strains showed that the positive rates of resistant genes mecA, Aac （6＇）/aph （2＂）, aph （3＇）-III, ermB, ermC and tetM were 100.0%, 93.8%, 76.9%, 7.7%, 96.9% and 4.6%, respectively; however, the resistant genes of ermA, tetO and vanA were negative. MRSH strains were classified into 3 types by RAPD and type A accounted for 83.1%. Conclusion There are high positive rate of resistant genes mecA, Aac（6＇）/aph（2）, aph（3＇）-Ⅲ and ermC in MRSH and type A strains are the most prevalent strains in the region.

关 键 词：溶血葡萄球菌 甲氧西林耐药 耐药基因 随机扩增多态性DNA技术 

分 类 号：R378.11[医药卫生—病原生物学]