抗黄矮病小麦新品系的分子标记检测研究  

Detection of wheat lines with resistance to Yellow dwarf virus by molecular markers

作　　者：王红梅[1] 王浩瀚 岳维云[3] 张正英[1] 欧巧明[1] 陈玉梁[1] 

机构地区：[1]甘肃省农业科学院生物技术研究所,兰州730070 [2]甘肃省张掖市农业科学研究所,张掖734000 [3]甘肃省天水市农业科学研究所中梁试验站,天水741000

出　　处：《植物保护》2012年第3期86-89,101,共5页Plant Protection

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2004-03;GNSW-2011-07)

摘　　要：本研究对以小麦-中间偃麦草异附加系L1和小麦-中间偃麦草部分双二倍体‘无芒中4’为抗源选育出的抗黄矮病小麦新品系进行分子检测和抗病性鉴定。通过应用RAPD、SSR、SCAR 3种分子标记OPF15、Xgwm37、SC-W37进行分子检测,并采用人工接种和大田自然感病的方式进行抗黄矮病鉴定,筛选到了‘93646’、‘2003-2’等高抗黄矮病的小麦新品系。分子检测抗黄矮病基因与田间抗病鉴定结果基本一致,应用的3种PCR标记都可以检测出抗病材料,但SCAR标记SC-W37特异性强、稳定性好,可在小麦抗黄矮病育种早代选择过程中发挥重要作用。Some BYDV translocation lines were studied by using wheat-Thinopyrum intermedium the addition line L1 and a partial amphiploid ＇Zhong 4 awnless＇ as the resistance parents. This translocation lines were used to screen molecular markers and identification of disease resistance. A RAPD marker OPF15, SSR marker Xgwm37 and SCAR marker SC-W37 were developed for the BYDV resistance. Some new strains with excellent BYDV resistance were selected by identification of disease resistance and molecular marker, including ＇2003-2＇ and ＇93646＇. The results showed that the BYDV resistant materials could be detected by PCR markers. The SCAR marker SC-W37 had high specificity and good reliability, which might play an important role in early-generation selection in wheat breeding.

关 键 词：小麦黄矮病 分子标记 检测 抗病鉴定 

分 类 号：S435.121.49[农业科学—农业昆虫与害虫防治]