气相色谱-三重串联四极杆质谱法测定粮谷中169种农药的残留量  

Determination of 169 Pesticide Residues in Grains by Gas Chromatography Tandem Mass Spectrometry

作　　者：郭新东 冼燕萍 罗海英 陈意光 侯向昶 罗东辉 吴玉銮 

机构地区：[1]广州市质量监督检测研究院国家加工食品质量监督检验中心广州,广州市食品安全检测技术重点实验室广州市食品安全风险动态监测与预警研究中心,广东广州510110

出　　处：《现代食品科技》2012年第6期695-702,共8页Modern Food Science and Technology

基　　金：广东省科技计划项目(2007B023001001);国家质检总局科技计划项目,液相色谱串联飞行时间质谱筛查检测水果中多农药残留的研究(2011QK326);广州市科信局科技计划项目,广州市食品安全检测技术重点实验室(穗科信字[2011]233-34号)

摘　　要：建立了粮谷中169种农药多残留的气相色谱-三重串联四极杆质谱(GC-MS/MS)的检测方法。样品经改进的QuEChERS净化处理,气相色谱分离,串联四级杆质谱多反应监测方式检测。结果表明,169种农药在粮谷中的检出限为0.3～13.0μg/kg,定量限为1.0～40.0μg/kg,线性范围在0.005 mg/L～2.0 mg/L之间,线性相关系数大于0.99,平均回收率在65.3%～143.6%之间,97%的农药相对标准偏差小于20%,该方法具有前处理简单、净化效果好、灵敏度高的特点,适用于粮谷中多组分农药残留的快速确认和定量检测。A method was developed for the simultaneous determination of 169 pesticides in grains by gas chromatography with tandem mass spectrometry （GC-MS/MS）. The homogenized sample was extracted with acetonitrile, and then purified by QuEChERS, The targeted compounds were detected by GC-MS/MS system under the mode of multiple reaction monitoring （MRM）. The results indicated that the limits of detection （LODs） of 169 pesticides were ranged from 0.3 to 13.0 ~tg/kg. The LOQs of the tested pesticides were between 1.0 and 40.0/lg/kg. In the linear range between 0.005 and 2.0 mg/L of each pesticide, the linear correlation coefficients were greater than 0.99. The average recoveries ranged from 65.3% to 143.6% and the RSDs of 97% pesticides were less than 20%. This method was suitable for the identification and quantification of multi-component pesticides in grains due to its simplicity, good purification and high sensitivity.

关 键 词：粮谷 农药多残留 气相色谱 三重串联四极杆质谱 

分 类 号：S481.8[农业科学—农药学]