骨质疏松大鼠脂肪基质细胞成骨能力的研究  

The Osteogenic Capability of the Adipose Derived Stem Cells from the Osteoporotic Rats

作　　者：申龙朵[1] 巩哈伲[1] 李嘉[1] 卢慧连[1] 李鹏[1] 汤炜[1] 

机构地区：[1]四川大学华西口腔医学院创伤与整形外科,四川成都610041

出　　处：《现代生物医学进展》2012年第15期2804-2809,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30973347;81170940);国家科技支撑计划资助项目(2009BA281B03)

摘　　要：目的:探讨骨质疏松大鼠的脂肪基质细胞有无成骨能力。方法:采用去势法构建SD大鼠骨质疏松模型,设置2组样本,分别为19月龄骨质疏松SD雌性大鼠,正常19月龄SD雌性大鼠。每组大鼠分别取腹股沟脂肪垫中脂肪,用胶原酶消化法培养获得脂肪间充质干细胞,绘制并分析细胞生长曲线;再用经典的骨向诱导液诱导2组ASCs,比较2组细胞骨向分化能力有无差别。结果:骨质疏松大鼠与正常老龄大鼠的ASCs骨向分化能力无明显差别。结论:可用去势法构建SD大鼠骨质疏松模型,并可取其细胞行进一步研究。老龄骨质疏松大鼠的ASCs也可以考虑作为种子细胞促进其自体骨愈合,从而为利用自体ASCs促进骨质疏松骨愈合打下一定的理论基础。Objective： To investigate if the ASCs of osteoporosis SD rat have the ability of osteogenic differentiation. Methods： In my study, the osteoporosis SD rat model was modeled by excising ovaries. We designed two test groups： 19 months female SD rats with osteoporosis, normal 19 months female SD rats. The fat tissue was obtained from the inguinal site. We got and cultured the ASCs by the tissue adherent and collagenase digestion methods independently to every group. And the rate was analyzed that getting ASCs successfully by each method. Also, we recorded the growth curves to illustrate the proliferation activity of the different groups＇ ASCs. On the other hand, the two ASCs were induced for osteogenesis through the classic ossifying culture solution; and the ability of osteogenic differentiation was compared. Results： There was no significant difference between the normal old rats and the osteoporotic ones. Conclusion： These results indicated that osteoporosis SD rat models could be constructed by ovariectomy. And the cells obtained from them were convenient for the follow-up cytology study. The ASCs from the old SD rats with osteoporosis could also be the seeding cells for enhancing the bone healing of themselves. And this conclusion offered an academic foundation for the treatment of bone fractures and defects using autologous ASCs.

关 键 词：脂肪间充质干细胞 骨向分化 骨质疏松 细胞培养 

分 类 号：R681[医药卫生—骨科学]