L-精氨酸和雨蛙素诱导急性胰腺炎模型的对比研究  

Comparison of the Model of Acute Pancreatitis Induced by L-arginine and Cerulein

作　　者：杨平[1] 寇明文[1] 赵戈[1] 卢鹏[1] 宋振顺[2] 

机构地区：[1]第四军医大学西京医院,陕西西安710032 [2]上海同济大学附属第十人民医院肝胆胰外科,上海200072

出　　处：《现代生物医学进展》2012年第15期2810-2813,共4页Progress in Modern Biomedicine

摘　　要：目的:研究L-精氨酸和雨蛙素分别诱导SD大鼠急性胰腺炎(AP)模型的差异,为进一步研究急性胰腺炎提供可靠模型。方法:L-精氨酸采用3次腹腔注射,间隔1 h,雨蛙素采用7次腹腔注射,间隔1 h诱导急性胰腺炎模型。碘-淀粉比色法检测血清淀粉酶水平,血清脂肪酶测定试剂盒检测脂肪酶活性,胰腺组织切片观察组织的破坏情况,TUNEL法检测腺泡细胞凋亡。结果:①L-精氨酸诱导的大鼠模型血清淀粉酶和脂肪酶水平在诱导成功后6 h即显著升高,蛙皮素诱导的大鼠模型在12 h显著升高,与正常对照组比较均有统计学差异(P<0.05),提示急性胰腺炎建模成功。②L-精氨酸诱导的模型中胰腺组织结构破坏,有大片出血坏死灶、大量炎细胞浸润;而蛙皮素诱导的模型组织腺泡、间质水肿,炎性细胞浸润,少量散在出血坏死灶,血管变化常不明显,渗液清亮。结论:L-精氨酸和雨蛙素均能诱导SD大鼠急性胰腺炎模型,L-精氨酸诱导重症急性胰腺炎,雨蛙素诱导轻型急性胰腺炎,是研究急性胰腺炎的良好模型。Objective： To investigate the difference of the models of acute pancreatitis（AP） induced by L-arginine and cerulein,so as to provide reliable models for further study of the acute pancreatitis.Method： Acute pancreatitis was induced in Sprague-Dawley rats either by giving three intraperitoneal injections of L-arginine,each at concentrations of 200 mg/100g body weight,with an interval of 1 h,or by giving seven intraperitoneal injections of cerulein,each at concentrations of 40 υg/kg body weight,with an interval of 1 h.Severity of AP was assessed by analysis of serum amylase and lipase activity.Samples of pancreas were taken for measuring hematoxylin-eosin staining.Apoptosis in pancreatic acinar cell was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique（TUNEL）.Result： ①Serum amylase and lipase activity has significantly increased at 6 h timepoint in the model of AP induced by L-arginine,and more higher with the extension of time.It has significantly increased at 12 h timepoint in the model induced by cerulein.Both have significantly difference（P〈0.05）.②The administration of L-arginine caused severe necrotizing pancreatitis,including large hemorrhage focal necrosis,and obvious inflammatory infiltration.However,there were edema of acinus and interstitial,a small amount of inflammatory cell infiltration in the model,which induced by cerulein.Conclusion： Acute pancreatitis were induced by L-arginine and cerulein.L-arginine could induce severely,and cerulein induce mildly.There both are feasible models of acute pancreatits for further research.

关 键 词：急性胰腺炎 L-精氨酸 雨蛙素 模型 

分 类 号：R332[医药卫生—人体生理学] R322.61[医药卫生—基础医学]