人外周血淋巴细胞中金属硫蛋白基因表达与其对镉易感性的关系  

Relationship between Metallothionein Gene Expression in Human Peripheral Lymphocytes and Their Susceptibility to Cadmium

作　　者：卢坚[1] 金泰廙 吴训伟[1] 

机构地区：[1]上海医科大学劳动卫生学教研室,200032

出　　处：《中国公共卫生》2000年第3期226-228,共3页Chinese Journal of Public Health

摘　　要：为探讨外周血淋巴细胞中金属硫蛋白基因表达作为对镉易感性生物标志物的可能性 ,采用分离人外周血淋巴细胞(PBL) ,体外培养 ,培养液中加入镉 ,检测接触镉后的细胞存活率、DNA、单链损伤、乳酸脱氢酶和金属硫蛋白 (MT)基因表达。结果 :体外接触 1 0 μmol/LCdCl2 6小时 ,细胞存活率在吸烟组和不吸烟组间无显著性差异。MT基因基础表达、诱导表达和诱导能力在组间未见差异。PBL中MT基础表达与LDH泄漏、彗星率和血镉水平呈显著相关 ,和细胞存活率则无相关。MT诱导能力与LDH泄漏和彗星率改变呈负相关。随MT基因诱导能力的上升 ,LDH泄漏和彗星率明显降低。提示PBL中MT基因诱导能力与其对镉的易感性有关 ,初步提示其作为镉接触的易感性标志物的可能性。To investigate the relationship between metallotinonein (MT) gene expression in human peripheral lymphocytes(PBL) and their susceptibility to cadmium. Methods: PBL was isolated and incubated with 10μm cadmium chloride for 6 hours. Cell viability, DNA single breakage, Lactate dehydrogenase (LDH)leakage and MT gene expression level were examined. Results: MT gene expression and cell viability did not show significant difference between smoking group and non smoking group. MT basal expression in PBL was correlated with LDH leakage, comet rate and ogrithm of cadmium concentration in blood (B Cd),but not with cell viability. MT inducible ability was negatively correlated with LDH leakage and comet rate. Conclusion: The results suggested the possibility of MT inducible ability as a biomarker of susceptibility.

关 键 词：镉 金属硫蛋白 淋巴细胞 基因表达 

分 类 号：R135.1[医药卫生—劳动卫生]