Actions of four organic acids in radix isatidis on endotoxin-neutralization investigated by kinetic turbidimetric assay  

作　　者：马莉 何英俊 李友 龚慕辛 

机构地区：[1]School of Traditional Chinese Medicine,Capital Medical University [2]Youcare Pharmaceutical Group Co.,Ltd [3]Patent Examination Cooperation of The Patent Office,SIPO

出　　处：《Journal of Traditional Chinese Medicine》2012年第2期261-266,共6页中医杂志（英文版）

基　　金：support of Nova of Beijing science and Technology (No. 2010B108);National Natural Science Fond (No. 30701091)

摘　　要：OBJECTIVE:To investigate anti-endotoxin action of four OAs reacted with endotoxin by the LAL assay with KTA.METHODS:Using a incubating kinetic tube reader and kinetic turbidimetric assay(KTA),the concentration-response time curve of endotoxin reacted with limulus amebocyte lysate(LAL) at 37℃ were obtained and the action of four organic acids(OAs) on it were investigated.The four OAs were benzoic acid,salicylic acid,syringic acid and 2-amino-benzoic acid from Radix isatidis.Meanwhile,the temperature variation caused by endotoxin with the four OAs was studied by the rabbit pyrogen test(RPT).RESULTS:It was showed that a low concentration(1 mg/mL) of the four OAs had a little effect of anti-endotoxin,and when the concentrations of the four OAs were 30 mg/mL,the endotoxin was neutralized completely.The relationships between the concentrations of endotoxin and the OAs were all linear with correlation coefficients of greater than 0.9995,indicating that the four OAs all had strong anti-endotoxin action,while syringic acid had the strongest action among the four OAs with IC50 of 12.84 mg/mL.CONCLUSION:The investigations of KTA agreed well with the results obtained by means of RPT.OBJECTIVE：To investigate anti-endotoxin action of four OAs reacted with endotoxin by the LAL assay with KTA.METHODS：Using a incubating kinetic tube reader and kinetic turbidimetric assay（KTA）,the concentration-response time curve of endotoxin reacted with limulus amebocyte lysate（LAL） at 37℃ were obtained and the action of four organic acids（OAs） on it were investigated.The four OAs were benzoic acid,salicylic acid,syringic acid and 2-amino-benzoic acid from Radix isatidis.Meanwhile,the temperature variation caused by endotoxin with the four OAs was studied by the rabbit pyrogen test（RPT）.RESULTS：It was showed that a low concentration（1 mg/mL） of the four OAs had a little effect of anti-endotoxin,and when the concentrations of the four OAs were 30 mg/mL,the endotoxin was neutralized completely.The relationships between the concentrations of endotoxin and the OAs were all linear with correlation coefficients of greater than 0.9995,indicating that the four OAs all had strong anti-endotoxin action,while syringic acid had the strongest action among the four OAs with IC50 of 12.84 mg/mL.CONCLUSION：The investigations of KTA agreed well with the results obtained by means of RPT.

关 键 词：Endotoxin Organic acid Kinetic turbidimetric assay Rabbit pyrogen test Radix isatidis 

分 类 号：R284[医药卫生—中药学]