血管紧张素Ⅱ的Ⅰ-型受体基因多态性与原发性高血压颈动脉硬化之间的关系  

The Study of the Relationship between Angiotensin Ⅱ Type 1 Receptor Gene Polymorphism and Carotid Artery Arteriosclerosis in Essential Hypertension

作　　者：柯晓刚[1] 林从容 吴可贵 林新霖[1] 谢良地 叶琼 

机构地区：[1]福建医科大学附属第一医院B超室,福建省福州市350005 [2]福建省高血压研究所

出　　处：《中国循环杂志》2000年第1期32-34,共3页Chinese Circulation Journal

基　　金：福建省科委基金!98A0 61

摘　　要：目的 :探讨血管紧张素 的 型受体 ( AT1 )基因多态性与原发性高血压颈动脉硬化之间的关系。　　方法 :盐析法提取原发性高血压组 ( n=12 0 )和正常对照组 ( n=86)的白细胞脱氧核糖核酸 ( DNA) ,采用多聚酶链反应结合限制性内切酶方法检测 AT1 基因 A1166C多态性 ;并进行正常对照组 ( n=3 2 )、原发性高血压组 ( n=68)的颈总动脉超声检测。　　结果 :原发性高血压组 AT1 基因的 AC基因型频率比正常对照组高 ( P<0 .0 1) ,C等位基因频率也高于正常对照组( P<0 .0 5 ) ;原发性高血压患者 AC基因型颈总动脉内膜中层复合体厚度和壁腔比值比 AA基因型患者大 ,而颈总动脉内径则无差别。　　结论 :AT1 基因 A1166C多态性与中国人原发性高血压有关 ,并且可能与原发性高血压颈动脉硬化有关。Objective:To investigate the relationship between polymorphism of angiotensin Ⅱ type 1 receptor (AT 1R) gene and carotid artery arteriosclerosis in essential hypertension(EH). Methods:Genomic deoxyribonucleic acid (DNA)was extracted from 86 normotensives and 120 hypertensives peripheral blood leukocytes by standard method.Intimal medial thickness、diameter、I/D of common carotid artery in 32 normotensives and 68 hypertensives were determined by ultrasound examination.Polymerase chain reaction (PCR) combined with restriction enzyme digestion was used to detect the polymorphism. Results:The frequencies of AC genotype and C1166 allele of AT 1R gene of EH patients were higher than those of control (AC genotype:0 181 vs.0 058, p <0 01,C allele:0 091 vs.0 029, p <0 05);EH subjects with AC genotype had incrcased common carotid artery intimal medial thickness and I/D compared with those with AA genotype. Conclusion:A1166C polymorphism of AT 1R gene was associated with EH in Chinese population and may play a role in arteriosclerosis of hypertension.

关 键 词：高血压 颈脉硬化 基因多态性 血管紧张素Ⅱ 

分 类 号：R544.1[医药卫生—心血管疾病] R543.4[医药卫生—内科学]