超极化停搏液对幼兔心肌缺血再灌注损伤的保护作用  

Protective Effects of Hyperpolarizing Cardioplegia on Immature Rabbits′ Myocardial Ischemic Reperfusion Injury

作　　者：王坚刚[1] 周新民[1] 唐浩 赵元[1] 胡野荣 

机构地区：[1]湖南医科大学附属第二医院心胸外科,湖南省长沙市410011

出　　处：《中国循环杂志》2000年第1期53-55,共3页Chinese Circulation Journal

摘　　要：目的 :研究钾通道开放剂所致超极化停搏对幼兔心肌缺血再灌注损伤的保护作用。　　方法 :在 L angendorff模型上分别用 St.Thomas 液 ( S组 ) ,St.Thomas 液加 5 0μmol/ L吡那地尔 ( S+P组 ) ,5 0μmol/ L吡那地尔 ( P组 ) 3种停搏液进行低温缺血再灌注离体心脏实验。观察停搏效果 ,心功能 ,冠状动脉血流量及心肌酶学等变化。　　结果 :再灌注后 P组心功能恢复明显优于其它两组 ,S+P组最差 ;P组冠状动脉流出液肌酸激酶含量最低 ,S+P组最高 ;再灌注 4 5分时 3组间冠状动脉血流量恢复率及心肌含水量无显著差异。　　结论 :超极化停搏液对未成熟心肌缺血再灌注损伤的保护作用优于去极化停搏液 ,但与 St.Thomas 液合用时保护作用差。Objective:We investigated the protective effects of hyperpolarizing cardioplegia by potassium channel agonist pinacidil on immature rabbits′ myocardial ischemic reperfusion injury. Methods:Twenty one hearts from NewZealand rabbits(aged from 21 to 28 days)were randomized to the groups of St.Thomas HospitalⅡSolution(group S),st.Thomas Hospital Ⅱ solution combinded with Pinacidil of 50 μmol/L(group S+P)and pinacidil of 50 μmol/L(group P)on the Langendorff Model to perform the in vitro hypothermic ischemic reperfusion test.The experimental index included cardioplgia efficiency,cardiac function,coronary blood flow and myocardial enzymes,etc. Results:Group P provided significantly better recovery of cardiac function than control groups,either did Group S than Group S+P;Group P demonstrated significant reduction of CK release in coronary outflow compared with control groups,and Group S+P showed the highest release,there were no significant differences among the 3 groups on the recovery rates of coronary flow and myocardial water contents. Conclusion:Compared with depolarizing St.Thomas Hospital Ⅱ Solution,the hyperpolarizing pinacidil solution provided more satisfactory myocardial protection on immature myocardial ischemic reperfusion injury,while pinacidil combinded with st.Thomas Hospital Ⅱ Solution makes unsatisfactory results.

关 键 词：心肌保护 超极化停搏液 心肌缺血 再灌注损伤 

分 类 号：R654.2[医药卫生—外科学] R542.2[医药卫生—临床医学]