^(147)Pm照射对人白血病HL-60细胞bc-l2和bax基因表达的研究  

Effect of ^(147)Pm β irradiation on bcl-2 gene and bax gene expression in human leukemia cells

作　　者：朱寿 彭肖东 

机构地区：[1]苏州医学院放射医学系,江苏215007

出　　处：《中华放射医学与防护杂志》2000年第1期33-35,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金!资助项目 (39470 389)

摘　　要：目的　研究了14 7Pm照射对人白血病HL 60细胞bcl 2和bax基因表达作用。　方法运用免疫组织化学技术探讨14 7Pm对HL 60细胞bcl 2 /bax的蛋白表达 ,以及RNA分子杂交技术探讨14 7Pm对HL 60细胞bcl 2mRNA的转录水平表达。结果　发现对照HL 60细胞中bcl 2蛋白呈高度表达 ,为 88% ;在14 7Pm照射下 ,可下调至 5 3 %。bax在对照细胞中的表达很低 ;14 7Pm作用后 ,未见明显改变。而受14 7Pm照射后 ,可使HL 60细胞中bcl 2mRNA表达呈明显下调 ,并随受照射时间的延长 ,下调作用更加显著。结论　14 7Pm诱发人白血病HL 60细胞的凋亡作用 ,与其下调凋亡相关基因bclObjective To study the effect of exposure to a pure β radiator\| 147 Pm on bcl 2 gene expression in human leukemia HL 60 cells. Methods Immunohistochemical technique was used to identify the expression of bcl 2 and bax proteins in HL 60 cells,as well as RNA dot blot hybridization to show the expression of bcl 2 mRNA in HL 60 cells. Results The expression of bcl 2 protein was as high as 88% in control human HL 60 cells. Down regulation of bcl 2 protein expression which lowered to 53% was noted after exposure to 147 Pm. The expression of bax protein was low in control cells, and after irradiation it did not obviously change. An obvious down regulation of bcl 2 mRNA expression was found in irradiated HL 60 cells. With increasing time of 147 Pm exposure, the down regulation of bcl 2 became more evident. Conclusion The apoptotic action of 147 Pm β rays in human HL 60 cells is associated with the down regulation of the apoptosis suppressing gene bcl 2.

关 键 词：HL-60细胞 基因表达 BCL-2 BAX 钷147 白血病 

分 类 号：R811.5[医药卫生—放射医学]