Tf-SEB偶合蛋白对荷瘤小鼠的疗效及其机理探讨  

An Initial Study of the Effect and Mechanism of Tf-- SEB Therapy with Coupled Protein for Mice Bearing Human Hepatoma

作　　者：张国驰[1] 陶箭[1] 胡宝瑜[1] 朱伯明 童善庆[1] 陆德源[1] 

机构地区：[1]上海第二医科大学微生物学教研室,上海200025

出　　处：《上海第二医科大学学报》2000年第2期105-107,131,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金!(39730270)

摘　　要：目的　研究人转铁蛋白（Ｔｆ）与金黄色葡萄球菌肠毒素Ｂ（ＳＥＢ）偶联后，在体内对肿瘤的治疗效果及其机理。方法　采用化学方法将Ｔｆ与ＳＥＢ偶联成Ｔｆ－ＳＥＢ偶联蛋白，观察该偶联蛋白对荷瘤小鼠的疗效；采用免疫细胞化学方法初步探讨该偶联蛋白在体内的抗肿瘤机理。结果　偶联蛋白处理组动物的肿瘤发生率和死亡率及肿瘤体积均显著低于单体ＳＥＢ处理组和其它各组；免疫细胞化学结果显示，偶联蛋白处理的动物肿瘤组织内有更为明显的ＣＤ４~＋Ｔ细胞和ＣＤ８~＋Ｔ细胞浸润。结论　经化学偶联的Ｔｆ－ＳＥＢ偶联蛋白在体内比单体ＳＥＢ具有更强的激活Ｔ细胞的抗肿瘤作用，该作用可能部分是由于具有导向效应的超抗原Ｔｆ－ＳＥＢ偶联蛋白诱导了超抗原依赖细胞介导的细胞毒效应所致。Objective To investigate the antitumor effect mechanism of transferrin(Tf) coupled with staphylococcus aureus enterotoxin B(SEB). Methods Tf was cross -- linked to SEB by the chemical method. The mice bearing human hepatoma were treated with Tf -- SEB coupled protein. The antitumor effect of Tf-- SEB and its antitumor mechanism were determined by immunocytochemistry. Results The percentage of the tumor generation, death and tumor size was respectively significantly lower in the group treated by Tf-- SEB than those groups treated by SEB or other agents. There were more CD8+ T cells and CD4+T cells in the tumor tissue of the group treated by Tf-- SEB than those groups treated by others. conclusion The chemically coupled protein, Tf-- SEB, was stronger than SEB to stimulate the T cells to produce antitumor cells, and this results might be due to the fact that Tf -- SEB induces the effect of superantigen -- dependent cell mediated cytotoxicity to the tumor cells.

关 键 词：转铁蛋白 Tf-SEB 超抗原 肿瘤 免疫疗法 

分 类 号：R392.12[医药卫生—免疫学]