丙型肝炎患者肝组织免疫组织化学研究  

Immunohistochemical findings on liver tissue in patients with hepatitis C

作　　者：陈宪锐[1] 玄梅香[2] 尹燕明[1] 李莉[3] 魏佑农[4] 赵新平 

机构地区：[1]泰山医学院传染病教研室,山东省泰安271000 [2]泰安市卫生防疫站 [3]济南市传染病医院 [4]新汶矿业集团中心医院 [5]枣庄市人民医院

出　　处：《中华流行病学杂志》2000年第2期128-129,共2页Chinese Journal of Epidemiology

摘　　要：目的　研究丙型肝炎 (丙肝 )病毒 (HCV)抗原在丙肝患者肝组织中的分布状况及其相互关系。方法　采取免疫组织化学方法 ,用多克隆抗 -HCV和单克隆抗 -HCV -NS3、抗 -HCV -NS5 ,对 10 7例丙肝患者肝组织中HCV抗原进行检测。结果　应用 3种抗体均从丙肝患者肝组织中检出相应抗原 ,检出率分别为 38.3%、2 8.0 %和 5 2 .3% ;阳性颗粒定位于肝细胞浆 ;阳性细胞分布呈散在、弥漫状和簇状 ;HCV -NS3的表达与炎症反应无明显解剖学关系 ,HCV -NS5阳性细胞较常见位于炎症灶旁或灶中。结论　HCV各成分在肝组织中的表达水平存在差别 ;HCVObjective To investigate the distributions and infer relations of HCV antigens in the liver tissues. Methods Immunohistochemical techniques as polyclonal antibody to HCV, monoclonal antibody to HCV-NS3 and monoclonal antibody to HCV-NS5 were used to detect HCV antigens in 107 patients with hepatitis C. Results HCV antigens in liver tissues were identified on these three kinds of antibodies. The positive rates were 38.3%,28.0% and 52.3% respectively. The positive granules were located in the cytoplasma of liver cells. The distributions of positive cells were scattered, diffusive and clustered. No obvious anatomic association was observed between the expression of HCV-NS3 and inflammatory reaction. HCV-NS5 positive cells were more commonly seen around or in the flammatory focci . Conclusion The expressions of various components of HCV were demonstrated in different concentrations. The expressions of HCV-NS5 might be related to the pathogenesis of hepatitis C.

关 键 词：丙型肝炎 HCV-NS3 HCV-NSS 抗原 免疫组化 

分 类 号：R512.63[医药卫生—内科学]