LC-MS/MS法测定人血浆中氨氯地平的浓度  

LC-MS/MS Determination of Amlodipine in Human Plasma

作　　者：王燕[1] 李鹏飞[1] 马萍[1] 安卓玲[1] 张茜[2] 罗恋[1] 刘丽宏[1] 

机构地区：[1]第二炮兵总医院药学部,北京100088 [2]湖北中医药大学,湖北武汉430065

出　　处：《质谱学报》2012年第3期181-185,共5页Journal of Chinese Mass Spectrometry Society

摘　　要：为了快速、准确地检测人血浆中氨氯地平的浓度,建立了高效液相色谱-质谱联用检测方法。选用Eclipse XDB-C18色谱柱,以甲醇-1mM乙酸铵溶液-0.1%甲酸溶液为流动相,采用梯度洗脱方法进行分离,样品经乙腈沉淀后进样,选用3200Q-trap型质谱仪的多重反应监测(MRM)扫描方式进行检测。氨氯地平线性范围为0.100～20.000μg/L,定量下限为0.100μg/L。准确度与精密度结果显示:方法日间、日内变异均小于15%,相对偏差为-3.85%～1.94%,低、中、高3个浓度提取回收率为94.90%～97.84%,稳定性好。该方法专属性强、样品处理简便、灵敏度高,可用于人体氨氯地平血药浓度的测定及其制剂的人体药代动力学研究。LC-MS/MS method was established for determination of amlodipine in human plasma. After protein precipitation of acetonitrile, the plasma sample was separated on an Eclipse XDB-C18 column using methanol -1 mM ammonium acetate-0. 1% formic acid as mobile phase by gradient elution. Detection was carried out by multiple reaction monitoring （MRM） on a 3200 Q-trap LC-MS/MS system. The assay was linear over the range 0. 100-20. 000μg/L with a lower limit of quantitation of 0. 100μg/L. Intra-day and inter-day precision were less than 15%, respectively. The relative deviation was in the range -3.85%- 1.94% The recovery of amlodipine was in the range 94.90%-97.84%. The method is a rapid, sensitive, selective and reliable method for the determination of amlodipine in human plasma. The assay can he applied for the determination of amlodipine in human plasma and the study on pharmacokinetics of its preparation.

关 键 词：高效液相色谱-质谱联用法 氨氯地平 人血浆 药代动力学 

分 类 号：O657.63[理学—分析化学]