系统性硬化症患者体内S100A12和sRAGE的表达及其临床意义  

Expression and clinical significance of S100A12 and sRAGE in vivo of patients with systemic sclerosis

作　　者：徐雪[1] 楚海燕[3] 梁敏锐[1] 吴文育[2] 王久存[3] 邹和建[1] 

机构地区：[1]复旦大学附属华山医院风湿科,上海200040 [2]复旦大学附属华山医院皮肤科,上海200040 [3]复旦大学生命科学院

出　　处：《临床内科杂志》2012年第5期320-323,共4页Journal of Clinical Internal Medicine

摘　　要：目的观察系统性硬化症（SSc）患者体内S100A12和可溶性晚期糖基化终产物受体（sRAGE）蛋白和基因表达水平，探讨这两种炎症性蛋自在疾病发病过程中的作用。方法选择肢端型SSc（1SSc）31例、弥漫型SSc（dSSc）40例和正常人50例，运用ELISA方法检测血浆中S100A12、sRAGE的含量，采用Real—timePCR法检测外周血细胞中这两个因子mRNA相对表达水平，并与临床表现和实验室指标进行相关分析。结果两种类型SSc患者血浆S100A12和sRAGE水平均高于对照组（P均〈0．05）。两种类型SSc患者血浆S100A12和sRAGE的浓度均呈正相关（r=0．583，P〈0．01；r=0．662，P〈0．01）。两种类型SSc患者外周血细胞S100A12mRNA相对表达量均高于对照组（P均〈0．05）。伴有肺部累及或肌肉累及的SSc患者血浆S100A12水平明显高于无上述器官累及者（P〈0．05）；伴有肺部累及或肾脏累及的SSc患者血浆sRAGE水平高于无上述器官累及者（P〈0．05）。抗U1RNP抗体、抗组蛋白抗体、抗RNA聚合酶Ⅲ抗体阳性的SSc患者血浆S100A12水平明显高于阴性组，而抗着丝点抗体阳性的SSc患者血浆SIOOA12和sRAGE水平均低于阴性组（P〈0．05）。结论S100A12和sRAGE在SSc患者体内表达显著升高，提示患者体内存在异常的炎症反应，且这两种蛋白含量与某些临床实验室指标有一定关系。Objective To investigate the protein and gene levels of S100A12 and sRAGE in the patients with systemic sclerosis, to analyze their clinical significances. Methods Thirty-one limited systemic sclerosis patients （1SSc） and forty diffuse systemic sclerosis patients （dSSc） were recruited. Plasma protein and mRNA levels of S100A12 and sRAGE in peripheral blood were measured by ELISA and realtime RT-PCR respectively. To further analyze the relations between SlOOA12/sRAGE and clinicolaboratory parameters in SSc patients. Results The plasma levels of S100A12 and sRAGE were significantly increased in dSSc and 1SSc patients as compared to the normal controls （ P 〈 0, 05 ） ; The levels of plasma S100A12 showed a positive correlation with the levels of plasma sRAGE in both 1SSc and dSSc patients. The relative mRNA levels of S100A12 were elevated in both kinds of patients compared to normal controis. The levels of plasma S100A12 were significantly increased in SSc patients with lung or muscle involvement than the patients without these organs involvement （ P 〈 0. 05 ） ; The levels of plasma sRAGE were significantly higher in SSc patients with lung or kidney involvement than the patients without these organs involvement. Meanwhile, the levels of plasma S100A12 were elevated in SSc patients with anti- U1RNP antibody positive or anti-histone antibody positive group or anti-RNA polymerase Ⅲ antibody positive group than these antibodies negative groups. Moreover, the levels of plasma S100A12 and sRAGE were significantly decreased in SSc patients with anti-centromere antibody positive group than antibody negative group. Conclusion Both S100A12 and sRAGE expression in SSc patients are significantly elevated suggests that the two protein may involve in the pathogenesis of scleroderma.

关 键 词：系统性硬化症 S100A12 可溶性晚期糖基化终产物受体 

分 类 号：R593.25[医药卫生—内科学]