海洋放线菌分离及菌株Streptomyces sp. AH17-3的次级代谢产物研究  

Isolation of marine actinomycetes and studies on the secondary metabolites of strain Streptomyces sp. AH17-3

作　　者：崔萌纳[1] 袭蕊 李德海[1] 顾谦群[1] 朱天骄[1] 

机构地区：[1]中国海洋大学医药学院海洋药物教育部重点实验室,山东青岛266003 [2]山东省章丘市妇幼保健院,山东章丘250200

出　　处：《中国海洋药物》2012年第3期1-6,共6页Chinese Journal of Marine Drugs

基　　金：国家海洋局公益专项(201005023);山东省科学技术发展计划(2009GG10002080资助)

摘　　要：目的对海洋放线菌进行分离及抗肿瘤活性筛选,并对一株具有抗肿瘤活性的海洋放线菌AH17-3的次级代谢产物进行研究。方法采用溶剂萃取、柱色谱层析及制备HPLC等方法对菌株AH17-3的发酵产物进行化学分离,通过理化性质及波谱学方法并参阅文献进行化合物结构鉴定,以SRB法评价化合物的抗肿瘤活性。结果从海洋样品中分离放线菌174株,从菌株AH17-3中分离得到了4个聚酮类化合物,经鉴定其结构分别为germicidin A(1)、germicidin B(2)、daidzein(3)、genistein(4)。其中化合物1具有弱的细胞毒活性,其IC50为3.5×10-7 M。结论海洋放线菌是重要的药用微生物资源,化合物1,2均为首次从海洋放线菌中分离得到。Objective To isolate actinomycetes strains from marine samples and study on the secondary metabolites of one marine-derived actinomycete. Methods The extract of the actinomyeetes was purified by solvent extraction, column chromatography and preparative HPLC. The structures were established by physicochemical properties and spectral analysis, and comparison with the data of literatures. The proliferation inhibition activities were evaluated by SRB method. Results The 174 aetinomycetes were i- solated from the marine samples, four polyketide compounds were identified as germicidin A （1）, germieidin B （2）, daidzein （3）, genistein （4）. And the compound 1 showed weak cytotoxic activities, its IC50 was 3.5×10^-7 M. Conclusion The marine-derived actinomycetes were important microbial resources of medicine, and compounds 1 and 2 were isolated from marine-derived actinomycetes for the first time.

关 键 词：海洋放线菌 次级代谢产物 细胞毒 

分 类 号：R915.1[医药卫生—微生物与生化药学] R965[医药卫生—药学]