海洋真菌zp6次级代谢产物及其抗真菌活性研究  

Secondary metabolites of marine fungus zp6 and their antifungal bioactivities

作　　者：高常松[1] 钟惠民[1] 曹俊伟[1] 陈颢[2] 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042 [2]国家海洋局第一海洋研究所海洋生物活性物质重点实验室,山东青岛266061

出　　处：《中国海洋药物》2012年第3期7-10,共4页Chinese Journal of Marine Drugs

基　　金：国家高技术研究发展计划项目(2007AA091905);国家自然科学基金项目(20872022);国家海洋局海洋生物活性物质重点实验室开放基金项目(MBS-2007-05)资助

摘　　要：目的对海洋真菌zp6发酵液的乙酸乙酯萃取部分及其抑菌活性进行研究。方法采用硅胶柱色谱及高效液相色谱进行分离纯化,根据理化性质及各种光谱技术进行结构鉴定,采用滤纸片法对分离到的化合物进行抑菌活性研究。结果从该菌中分离纯化得到8个化合物:(22E)-3β,5α,9α-三羟基-麦角甾-7,22-二烯-6-酮(1)、5α,8α-环二氧-24-甲基麦角甾-6,22-二烯-3β-醇(2)、赤藓糖醇(3)、腺嘌呤核苷(4)、(24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(5)、阿拉伯醇(6)、(7)、4-乙酰氧基苯甲酸(8)。结论化合物1和5具有抗真菌活性。Objective The secondary metabolites of marine fungus zp6 and their antibacterial activities were studied. Methods The compounds were isolated by means of silica gel column and HPLC chromatography. Their structures were determined on the basis of physicochemical evidences and spectral methods. In order to evaluate the antifungal activity of the compounds, filter paper method was used. Results Eight compounds were isolated from the fermentation liquid extracted by ethyl acetate, and were identified as （22E）-3β 5α, 9α-trihydroxy-ergosta-7, 22-dien-6-one（1）, 5α, 8α-epidioxy-ergosta-6, 22-dien-3ffol （2）, erythritol （3）, adenosine（4）, （24R）-ergosta-7, 22-diene-3β, 5α, 6β-triol （5）, ara binitol （6）, 9-octadecenoic acid （7）, 4-acetoxybenozic acid （8）. Conclusion Compounds 1 and 5 showed antifungal activities.

关 键 词：海洋真菌 代谢产物 抗真菌活性 

分 类 号：Q939.11[生物学—微生物学] R917[医药卫生—药物分析学]