钯(Ⅱ)-泛昔洛韦螯合物与铬天青S相互作用的共振瑞利散射光谱及其分析应用  

Study on the Interaction of Pd(Ⅱ)-Famciclovir with Chrome Azurol S by Resonance Rayleigh Scattering Spectra and Their Analytical Application

作　　者：张敏[1] 吴利敏[1] 周尚[2] 杨季冬[1,3] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]长江师范学院化学化工学院,重庆408100 [3]重庆三峡学院化学与环境工程学院,重庆404100

出　　处：《分析测试学报》2012年第6期633-638,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21175015);教育部春晖计划(Z2009-1-63003)

摘　　要：在pH值为2.5～3.5的Britton-Robinson缓冲溶液中,泛昔洛韦与钯(Ⅱ)相互作用形成1∶1的螯合阳离子,并进一步与铬天青S反应形成1∶1的离子缔合物。该反应可引起共振瑞利散射(RRS)光谱的显著增强并产生新的RRS光谱,最大RRS波长位于367 nm。在一定范围内,共振瑞利散射增强(ΔIRRS)与泛昔洛韦的质量浓度成正比,其线性范围为0.02～2.4 mg/L。该方法的灵敏度高,对泛昔洛韦的检出限为3.6μg/L。实验考察了适宜的反应条件以及共存物质的影响。应用计算化学软件Gaussview3.07和Gaussian03W,采用密度泛函法,在B3LYP/6-31G基组水平上计算了泛昔洛韦的电荷分布,对反应机理和RRS增强的原因进行了讨论。基于Pd(Ⅱ)-泛昔洛韦-铬天青S体系三元离子缔合物的RRS光谱,发展了一种简便、快速、灵敏测定泛昔洛韦的新方法。此方法用于胶囊和尿样中泛昔洛韦的测定,结果满意。In Britton - Robinson buffer of pH 2. 5 - 3.5, famciclovir（FCV） could react with palladium （Ⅱ） to form 1 ： 1 anionic chelates. The chelates react further with chrome azurol S（CAS） to form the ion - association complexes, which could result in the great enhancement of resonance Rayleigh scattering （RRS） intensities and a new RRS spectra appearing. The maximum RRS wavelength locates at 367 nm. Under the optimum conditions, the intensities of RRS are proportional to concentration of FCV in the range of 0. 02 - 2. 4 mg/L, with a detection limit of 3.6 μg/L. The reaction conditions of the system and effect of coexisting substances were investigated. The softwares of Gaussview 3.07 and Gaussian 03W at a B3LYP/6 -31 G （base group）level based on the density functional theory （DFT） were used to calculate the charge distribution of FCV. The reaction mechanism and the reasons for RRS enhancement of the system were investigated. The method was simple, rapid and sensitive , and was applied in the determination of FCV in capsule and urine samples with satisfactorily result.

关 键 词：共振瑞利散射 泛昔洛韦 钯(Ⅱ) 铬天青S 

分 类 号：O561.3[理学—原子与分子物理] TQ460.72[理学—物理]