茶叶中吡虫啉农药残留的液相色谱检测方法  

A Method for Determination of Imidacloprid Residue in Tea with HPLC-UV

作　　者：王孝辉[1] 宛晓春[1] 侯如燕[1] 

机构地区：[1]安徽农业大学教育部、农业部茶叶生物化学与生物技术重点开放实验,安徽省食品安全分析与测试重点实验室,安徽合肥230036

出　　处：《茶叶科学》2012年第3期203-209,共7页Journal of Tea Science

基　　金：农业部现代农业产业技术体系(农科教发[2008]10号);2010年农业行业标准制定和修订项目(农财发[2010]49号)

摘　　要：建立了茶叶中吡虫啉农药残留的液相检测方法。对比了几种常用溶剂和提取方式的提取率以及固相萃取材料对茶叶样品中干扰基质的净化效果。研究结果表明，茶叶经水浸泡，乙腈高速匀浆提取，Envi-Carb／PSA固相萃取柱净化，乙腈6mL洗脱后，经反相高效液相色谱紫外检测器检测茶叶中毗虫啉农药的残留量，吡虫啉的添加质量分数为0．025-0．250mg／kg，其回收率为92．0％-97．6％，相对标准偏差（RSD）≤3．8％，方法的定量限为0．025mg／kg，符合出口茶叶吡虫啉农药残留分析要求。前处理方法简单快捷，有机溶剂用量少。A method for the analysis of imidacloprid residue in tea by high performance liquid chromatography （HPLC） with UV detector was established. Through the comparation of extract efficiencies of several solvent and the clean-up capacities of different SPE cartridges, the results show that after soaked with water, extracted by acetonitrile, cleaned up with the Envi-Carb/PSA cartridge, imidacloprid was eluted with 6 mL acetonitrile solution from the cartridge and detected with HPLC, the samples was spiked at the level of 0.025-0.250 mg/kg, and the recovery of Imidacloprid range from 92.0%-97.6% with RSD less than 3.8%. Practical determination limit was 0.025 mg/kg. It meet with the analytical requirements to imidacloprid residue in exported tea. The pretreatment method was simple, sensitive and less organic solvent consumption.

关 键 词：茶叶 吡虫啉 农药残留 液相色谱 

分 类 号：S481.8[农业科学—农药学]