太原地区急性呼吸道感染病毒病原谱研究  

Viral pathogenic spectrum of acute respiratory infection in Taiyuan

作　　者：任斌知[1] 张杰敏[1] 郭支喜[1] 王乃昌[1] 赵嵘[1] 张凡非[1] 冯军军[1] 李国华[1] 高雪芬[1] 左素俊[1] 

机构地区：[1]山西省疾病预防控制中心疾病检验科,太原030012

出　　处：《中国药物与临床》2012年第6期708-711,共4页Chinese Remedies & Clinics

基　　金：山西省卫生厅科技攻关项目(200848);山西省留学回国人员科技活动项目(2008年度)

摘　　要：目的了解太原地区急性呼吸道感染(ARI)病例的病毒病原学特性及分布情况,为ARI疾病的预防控制和治疗提供科学依据。方法利用荧光定量聚合酶链式反应(PCR)方法,对381份上呼吸道和下呼吸道ARI病例的呼吸道样本进行了流感病毒(IFV-A、H1、H3、甲型H1N1、B型、C型)、呼吸道合胞病毒(RSV-A、B)、人副流感病毒(HPIV-1～4型)、人冠状病毒(HCoV-229E、OC43、NL63、HKU1型)、人偏肺病毒(hMPV-A、B)、人博卡病毒(HBoV)、呼吸道腺病毒(R-ADV)等7种病毒(共计20个型别)的检测,并对结果数据进行了统计分析。结果 381份样本中病毒检测阳性结果有164份,阳性率为43.0%,分别为IFV(22.8%)、RSV(10.8%)、hMPV(3.7%)、HPIV(2.9%)、HCoV(2.6%)、R-ADV(1.8%)、HBoV(0.8%),其中同时2种以上病毒混合感染8份,占4.9%。全年中均有病毒感染病例被检出,主要集中在冬春季,IFV、RSV、hMPV阳性率最高月份分别为12月(71.4%)、2月(84.2%)、1月(33.3%)。另外,上呼吸道ARI病例样本323份,病毒阳性率为34.4%,以IFV为主(占76.6%);下呼吸道ARI病例样本58份,病毒阳性率为91.4%,以RSV为主(占75.5%);ARI病例中15岁以下儿童占有较大比例,其中3岁以下患儿的病毒感染率为85.7%。结论 IFV、RSV、hMPV为太原地区2009-2011年ARI病例的主要致病原,并且各病毒随年龄、流行季节中月份、感染部位等不同而有各自的流行特性。Objective To investigate viral pathogenic characters and distribution of acute respiratory tract infection （ARI） in Taiyuan, thereby offering scientific foundation for prevention and control of ARI. Method We detected the viruses and analyzed data in a cohort of 381 respiratory tract specimens sampled from patients with upper or lower ARI by using fluorescence real-time polymerase chain reaction. The 7 categories of viruses （20 subtypes in total） were ordered as follows： influenza virus （IFV-A, H1, H3, type A HIN1, type B and type C）, respiratory syncytial virus （RSV-A and B）, human parainfluenza virus （HPIV type 1-4）, human coronavirus （HCoV-229E, OC43, NL63 and HKU1）, human metapneumovirus （hMPV-A and B）, human bocavirus （HBoV） and respiratory adenovirus （R-ADV）. Results Of 381 samples for viral detection, 164 （43.0%） tested positive, with the presence ordered as IFV （22.8%）, RSV （10.8%）, hMPV （3.7%）, HPIV （2.9%）, HCoV （2.6%）, R-ADV （1.8%） and HBoV（0.8%）, respectively. There were 8 samples （4.9%） shown as having coinfection involving at least 2 types of viruses. Viral infection could be detected throughout the year, particularly, in winter and spring. IFV, RSV and hMPV were found as exhibiting peak positive rate in December （71.4%）, February （84.2%） and January （33.3%）, respectively. In addition, 34.4% tested positive out of 323 samples from patients with upper ARI, showing predominance of IFV infection （76.6%）. In contrast, 91.4% detected positive out of 58 samples in those with lower ARI, suggesting RSV-predominated infection （75.5%）. Children aged less than 15 years accounted for the majority of ARI cases. This was most profound in children under 3 years of age （85.7%）. Conclusion IFV, RSV and hMPV comprise of the leading viral pathogens in patients with ARI in Taiyuan between 2009 and 2011. These viruses may be characterized of unique epidemiological feature depending upon subject＇s age, month of epidemic sea

关 键 词：呼吸道感染 病毒 病原 荧光定量PCR法 

分 类 号：R56[医药卫生—呼吸系统]