早食李离体胚培养体系的建立  

Establishment of in vitro embryo culture system in Prunus salicina cv.zaoshi

作　　者：谢志亮[1,2] 吴振旺[1] 彭兵[2] 何业华[2] 

机构地区：[1]温州科技职业学院,浙江温州325006 [2]华南农业大学园艺生物技术研究所,广东广州510640

出　　处：《中南林业科技大学学报》2012年第5期76-79,共4页Journal of Central South University of Forestry & Technology

基　　金：公益性行业(农业)科研专项(No.201003058)资助

摘　　要：以早食李胚为研究材料,分析了基本培养基、6-BA、IBA、种皮以及AgNO3、GA3、CH、AC等几种添加物对李胚离体培养的影响,初步建立了早食李胚离体培养体系。李胚萌发成苗培养基为F14+6-BA 0.5mg/L+IBA 0.3mg/L+琼脂7g/L+3%蔗糖,并可作为壮苗培养基;丛生芽增殖培养基为F14+6-BA 1-2mg/L+IBA 0.3mg/L+琼脂7g/L+3%蔗糖;生根培养基为1/2MS+IBA 3mg/L+琼脂7g/L+3%蔗糖。其中,早食李胚种皮的剥除与否是其萌发的关键。单独添加AgNO3、GA3、CH、AC等4种附加物对早食李的萌发成苗率没有作用,而同时添加却能提高其成苗率。By taking Prunus salicina cv. zaoshi as testing materials, the effects of various additives such as basic medium, 6-BA, IBA, seed capsule, AgNO3, GA3, CH and AC on in vitro embryo culture of P salicina cv. zaoshi were studied, and the system of mature P salicina cv. zaoshi embryo culture was established. The optimal medium for germination and seedling was F14＋6-BA 0.5 mg/L＋IBA 0.3mg/L＋sugar 3%＋agar 7g/L, which also was suitable for strong seeding; The optimal medium for shoot generation was F14 ＋6-BA 1-2mg/L＋IBA 0.3mg/L＋sugar 3%＋agar 7g/L; The optimal medium for rooting was 1/2MS＋IBA 3mg/L＋sugar 3%＋agar7 g/L; While the decorticate of seed capsule was key factor which affected the germination of P salicina cv. zaoshi embryo. The accessories of AgNO3, GA3, CH and AC that were only added to the additives were no effect on germination at all, but that all four accessories were added to the additives could increase the seeding rate.

关 键 词：早食李 胚培养 不定芽诱导 增殖 

分 类 号：S722.19[农业科学—林木遗传育种] S662.3[农业科学—林学]