披碱草内生真菌共生体中麦角生物碱的组织分布与季节动态  

Distribution and seasonal dynamics of ergot alkaloids in Elymus dahuricus-endophytic fungus symbiont

作　　者：徐瑞[1] 南志标[1] 周雁飞 李春杰[1] 

机构地区：[1]兰州大学草地农业科技学院草地农业生态系统国家重点实验室,甘肃兰州730020

出　　处：《草业学报》2012年第3期84-92,共9页Acta Prataculturae Sinica

基　　金：国家"973"项目(2007CB108902)资助

摘　　要：采用高效液相色谱(HPLC)检测了2009年4,6,8和10月采集的雾灵山、沁源、五台山和百望山等4个侵染了Neotyphodium属内生真菌的披碱草种群的叶片、茎、叶鞘和根以及10月采集的种子中2种麦角生物碱(麦角酰胺和麦角新碱)的含量。结果表明,在4,6和8月,4个披碱草种群的麦角酰胺主要分布在叶片(5.037～30.037mg/kg)和叶鞘(6.259～22.255mg/kg),10月,麦角酰胺主要集中在种子(5.045～8.971mg/kg)和叶片(3.119～6.658mg/kg);各种群的叶片、茎秆和叶鞘中麦角酰胺在4或6月含量(13.831～30.037mg/kg)最高,8或10月含量(3.119～6.658mg/kg)显著减小(P<0.05),根中8月麦角酰胺(0.552～0.576mg/kg)显著低于其他月份(P<0.05)。麦角新碱含量的分布与麦角酰胺相似,4,6和8月主要分布于叶片(2.206～8.226mg/kg)和叶鞘(0.268～5.490mg/kg)中,10月主要集中于种子(1.647～5.664mg/kg)和叶片(1.353～2.251mg/kg)中;各种群的叶片、茎秆和叶鞘中麦角新碱在4或6月(5.102～8.226mg/kg)最高,8或10月含量(1.353～2.251mg/kg)显著减小(P<0.05),根中麦角新碱在8月含量(0.128～0.283mg/kg)低于其他月份。不同生长时期,4个披碱草种群整株中麦角酰胺和麦角新碱的含量均不同。麦角生物碱的总含量中麦角酰胺显著高于麦角新碱(P<0.05),麦角酰胺和麦角新碱呈极显著线性正相关(P<0.01)关系,相关系数为r=0.827。Ergine and ergonovine contents in leaf blades,pseudostems,leaf sheaths,roots and seeds of four populations of Elymus dahuricus infected by grass endophyte（Neotyphodium） from Wulingshan（WLS）,Qinyuan（QY）,Wutaishan（WTS） and Baiwangshan（BWS）,were detected in April,June,August and October of 2009 using high performance liquid chromatography.In April,June,and August the highest contents of ergine were detected in leaf blades（5.037-30.037 mg/kg） and sheaths（6.259-22.255 mg/kg）,while in October the highest values were found in the seeds（5.045-8.971 mg/kg） and leaf blades（3.119-6.658 mg/kg）.Ergine contents in leaf blades,pseudostems and leaf sheaths in April or June（13.831-30.037 mg/kg） were significantly higher than those in August or October（3.119-6.658 mg/kg）.Ergine contents in the roots in August（0.552-0.576 mg/kg） were significantly lower than those in other months.The distributions of ergonovine in different tissues showed a similar pattern to those of ergine.The highest contents of ergonovine were detected in leaf blades（2.206-8.226 mg/kg） and sheaths（0.268-5.490 mg/kg） in April,June and August,while in October the highest values were also found in the seeds（1.647-5.664 mg/kg） and leaf blades（1.353-2.251 mg/kg）.Ergonovine contents in leaf blades,pseudostems and leaf sheaths in April or June（5.102-8.226 mg/kg） were significantly higher than those in August or October（1.353-2.251 mg/kg）.The lowest contents of ergonovine were found in August（0.128-0.283 mg/kg）.The contents of ergine and ergonovine in the whole plant of these four populations were different in different months.Ergine contents were significantly higher than ergonovine contents in total ergot alkaloids.Ergine contents were significantly linearly correlated with ergonovine contents.

关 键 词：披碱草 内生真菌 麦角生物碱 分布 季节动态 

分 类 号：S543.9[农业科学—作物学]