玉米ZmCBL2-2基因的克隆及其表达分析  

Cloning and Expression Analysis of ZmCBL2-2 Gene from Maize(Zea mays. L)

作　　者：李记园[1] 王存芳[1] 赵欢欢[1] 王茅雁[1] 

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

出　　处：《华北农学报》2012年第2期30-34,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30760120)

摘　　要：为通过转基因植物等方法分析玉米ZmCBL2-2基因在耐逆性中的功能,运用RT-PCR(Reverse transcriptionPCR)方法克隆了该基因的编码区cDNA片段,并将其构建到植物表达载体p3301(pCAMBIA3301)上。同时,运用荧光实时定量PCR(Real-time quantitative PCR,RT-qPCR)技术对玉米幼苗期该基因在高盐、低温和养分缺乏等6种非生物胁迫下的表达动态进行了分析,发现其对于缺N、低K、低温、外源ABA(Abscisic acid)和水分胁迫均有显著的应答反应,而对于盐胁迫无明显的响应。To analyze the role of ZmCBL2-2 gene in the tolerance of maize to abiotic stresses by methods such as transgenic plants,the complete coding region cDNA fragment of the gene was cloned by reverse transcription pol- ymerase chain reaction （RT-PCR） and constructed into the plant expression vector p3301 （pCAMBIA3301）. More- over, the expression profiles of ZmCBL2-2 under six abiotic stresses such as high salinity,low temperature and nutri- tional deficiency were analyzed by real time quantitative PCR （RT-qPCR） method,and it was found that the gene responded significantly to nitrogen deprivation,low potassium,low temperature, exogenous abscisic acid （ABA） and water stress, hut did not respond obviously to salt stress.

关 键 词：玉米 CBL 基因克隆 表达分析 

分 类 号：S513[农业科学—作物学] Q78[生物学—分子生物学]