甜瓜乙烯受体基因Cm-ETR2的克隆及其在果实发育成熟过程中表达特性的分析  

Cloning and Expression Characterization of Ethylene Receptor Gene Cm-ETR2 from Melon(Cucumis melo L.)

作　　者：郝金凤[1] 王雪飞[1] 怡荣[1] 哈斯阿古拉[1] 牛一丁[1] 

机构地区：[1]内蒙古大学生命科学学院,内蒙古自治区牧草与特色作物生物技术重点实验室,内蒙古呼和浩特010021

出　　处：《华北农学报》2012年第2期40-43,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金资助项目(30960159);高等学校博士学科点专项科研基金(200801260002);教育部留学回国人员科研启动基金资助

摘　　要：为研究Cm-ETR2基因在甜瓜果实发育成熟过程中的作用机理,根据已报道的甜瓜(Cucumis melo)品种Cantalupensis的Cm-ETR2基因序列,设计特异性引物,通过RT-PCR技术从甜瓜品种河套蜜瓜(Cucumis melo L.cvHetao)成熟果实中克隆得到了乙烯受体基因Cm-ETR2 cDNA,序列分析显示,序列长度为2 304 bp,编码767个氨基酸。利用实时荧光定量RT-PCR方法,对其在河套蜜瓜果实发育成熟过程中的表达特性进行了分析,Cm-ETR2基因在15DAP至30DAP表达基本没有变化,35DAP表达量开始上升,上升趋势一直持续至45DAP,且45DAP表达量为15DAP的9倍。A pair of specific primer was designed based on melon ethylene receptor gene Cm-ETR2 in Genbank.The full-length cDNA of Cm-ETR2 was cloned by RT-PCR from ripening fruit of melon（Cucumis melo L.cv.Hetao）.The length of cDNA was 2 304 bp,and the cDNA encodes 767 amino acids.Sequence analysis indicated that the cDNA sequence shared high similarity to that of the melon ETR2 reported previously.And the deduced amino acid sequences are consistent with those of the melon reported previously.The expression characterization of these genes during melon fruit development and ripening was analyzed by real-time quantitative RT-PCR.The results indicated that there were no changes of the expression level of ETR2 from 15 DAP to 30 DAP.The expression level began to increase at 35 DAP and the increasing continued until 45 DAP.The highest level was 9 times than 15 DAP.

关 键 词：甜瓜 乙烯受体 ETR2 果实成熟 表达 

分 类 号：S652.1[农业科学—果树学]