人工异源六倍体小麦中甲基化差异的MSAP分析  

MSAP Analysis of DNA Methylation Alteration in Synthetic Hexaploid Wheat

作　　者：聂利红[1] 王延召[1] 孙其信[2] 房卫平[1] 谢德意[1] 

机构地区：[1]河南省农业科学院经济作物研究所,河南郑州450002 [2]西北农林科技大学,陕西杨凌712100

出　　处：《华北农学报》2012年第2期77-80,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30370878)

摘　　要：利用MSAP方法分析了小麦从四倍体到六倍体进化过程中甲基化水平和甲基化遗传模式的变化。结果表明,人工异源六倍体小麦SCA/SQ(AABBDD)及其四倍体母本SCAUP(AABB)、二倍体父本粗山羊草SQ523(DD)的总甲基化水平分别为28.21%,27.53%,24.11%。37对引物检测到1 087条差异的扩增片段,对这些位点的甲基化遗传模式进行分析,结果表明,在人工异源六倍体中发生过甲基化的比例为18.95%,高于去甲基化的比例(2.58%)。这些结果揭示了小麦从四倍体到六倍体的进化过程中,通过对一些功能基因的甲基化来减轻异源多倍化造成的影响。DNA methylation is known to play an important role in the regulation of gene expression in higher plants.In this study,we assessed the extent and patterns of cytosine methylation alterations in a stable synthetic hexaploid wheat and their progenitor parents using MSAP.Results showed that the levels of methylation in SCA/SQ（AABBDD） and the parents were 28.21%,27.53%,24.11%,respectively.A total of 1 087 fragments were differentially amplified from the two digests for at least one of the parental lines,about 21.53% of loci shown changes in methylation patterns in the allopolyploid.Among these loci,18.95% were hypermethylated,2.58% were demethylated.The increased methylation level in synthetic hexaploid wheat compared to its parents might mitigate the shocks from the hybridization and duplication.

关 键 词：人工六倍体小麦 异源多倍化 甲基化 甲基化敏感扩增多态性 

分 类 号：S512.01[农业科学—作物学]