拮抗大豆根腐病细菌的Biolog鉴定  

Identification of Biocontrol Bacteria against Soybean Root Rot with Biolog Automatic Microbiology Analysis System

作　　者：许艳丽[1] 刘海龙 李春杰[1] 潘凤娟[1] 李淑娴 刘新晶[4] 

机构地区：[1]中国科学院东北地理与农业生态研究所黑土区农业生态院重点实验室,黑龙江哈尔滨150081 [2]黑龙江倍丰农业生产资料集团有限公司,黑龙江哈尔滨150020 [3]United States Department of Agriculture-Agricultural Research Service(USDA/ARS),Crop Genetics Research Unit(CGRU),Stoncville,MS 38776,USA [4]黑龙江生态工程职业学院,黑龙江哈尔滨150025

出　　处：《华北农学报》2012年第2期234-238,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家“十一五”科技支撑计划项目(2006BAD21B01-15);黑龙江省“十一五”科技攻关项目(GA06B101-1-5)

摘　　要：为明确2株生防细菌的分类地位,采用传统形态学方法结合Biolog微生物自动分析系统,鉴定了大豆根腐病的2株生防细菌。结果表明,菌株B021a与塔式弧菌相似度值为0.634,可能性是86%,遗传距离为4.00。菌株B04b与海藻巴斯德菌相似度值为0.610,可能性是75%,遗传距离为2.77。综合形态学和Biolog鉴定结果,认为菌株B021a是塔式弧菌,菌株B04b是海藻巴斯德菌。In order to identify the systematic position of taxonomy of two biocontrol bacteria against soybean root rot. Traditional morphological identification and BIOLOG automatic microbiology analysis system were used to iden- tify strain BO21a and B04b. The results showed that similarity value of strain B021a with Vibrio tubiashii was 0. 634, possibility to 86% and genetic distance to 4.00, and similarity value of strain B04b with PasteureUa trehalosi was 0.610,probability to 75% and genetic distance to 2.77. Strain BO21a was identified as Vibrio tubiashii and strain B04b as Pasteurella trehalosi by colony morphological propertie and BIOLOG analysis system.

关 键 词：大豆 根腐病 拮抗细菌 Biolog自动分析系统 鉴定 

分 类 号：S432[农业科学—植物病理学]