培美曲塞腹腔用药对荷H22腹水瘤小鼠腹水抑制作用及其毒副反应的实验研究  

Inhibition Effect and Toxicity of Intraperitoneal Administration of Pemetrexed on H22 Ascites Tumor-bearing Mice

作　　者：潘琦津[1] 李永强[2] 胡晓桦[2] 刘志辉[2] 廖小莉[2] 林燕[2] 

机构地区：[1]广西医科大学研究生学院 [2]广西医科大学附属肿瘤医院,南宁市530021

出　　处：《广西医学》2012年第5期524-528,共5页Guangxi Medical Journal

基　　金：广西医药卫生科研课题(重200869)

摘　　要：目的探讨培美曲塞腹腔用药对未添加叶酸荷H22肝癌腹水瘤昆明小鼠的疗效与安全性。方法雄性昆明小鼠腹腔内注射密度为1×107/ml的H22瘤细胞悬液0.2 ml(含H22细胞数2×106),制备恶性腹水模型。小鼠造模后随机分为6组,每组9只:正常对照组(生理盐水),模型对照组(生理盐水),培美曲塞低、中、高剂量组(10、50、90 mg.kg-1.d-1),阳性对照组(顺铂0.6 mg.kg-1.d-1)。造模24 h后给予各药物腹腔内注射治疗,用药时间为第1～5天、第8～12天。每天用药前测量小鼠的体重、腹围,并观察其日常生活状态,第13天处死各组小鼠并测量腹水量和血常规。结果与模型对照组比较,不同剂量的培美曲塞均可抑制荷H22腹水瘤KM小鼠腹水的生成(P<0.01),但出现较明显的毒副反应,血常规异常,并有小鼠死亡。结论培美曲塞腹腔用药可抑制未添加叶酸荷H22腹水瘤KM小鼠腹水的形成并促进其消退,但同时出现严重的血液系统毒副反应。Objective To observe the effect and safety of intraperitoneal administration of pemetrexed （PEM） on H22 ascites tumor-bearing Kunming（KM） mice that had not supplemented folic acid. Methods The mice model of ascites was established by intraperitoneal injection with suspension, density of 1 × 107/ml, of 0.2 ml（2 ×106 H22 cells）. And then animals were subsequently divided into 6 groups randomly（9 mice per group）：normal control group （saline） ;model control group（saline）;PEM low,middle,high dose group（ 10,50,90 mg · kg^-1 · d-＇）;positive control group（DDP,0.6 mg · kg^-1 ·d-1 ）. All experimental mice were injected intraperitoneally with drug from the 1st day to the 5th day,and the 8th day to the 12th day,respectively. The body weights,abdomen circumference of the mice were measured and their behavior was observed every day before treatment. All the experimental animals were sacrificed, and their ascites volumes and the results of blood routine examination were recorded on the 13th day. Results Compared with model control group, various doses of PEM could inhibit the production of ascites in H22 ascites tumor-bearing KM mice（P 〈0.01 ）,but showed significant toxic side effects,and the abnormalities could be found in the blood routine examination and some mice died. Condusion Intraperitoneal administration of PEM for I-I22 ascites tumor-bearing KM mice that had not supplemented folic acid has good therapeutic effect on delaying ascites regeneration and subsidizing it, but also has a serious hematologic toxicity.

关 键 词：培美曲塞 腹水 腹腔内 药物疗法 动物模型 

分 类 号：R442.5[医药卫生—诊断学] R73-3[医药卫生—临床医学]