HPLC法测定金钱利胆丸中丹酚酸B的含量  

Determination of Salvianolic Acid B Content in Jinqian Lidan Pill by HPLC

作　　者：张府君[1] 程永杰[1] 杨兴鑫[1] 李晓妮[1] 

机构地区：[1]山西医科大学药学院,山西太原030001

出　　处：《山西中医学院学报》2012年第2期36-38,共3页Journal of Shanxi College of Traditional Chinese Medicine

摘　　要：目的:建立金钱利胆丸中丹酚酸B含量的测定方法。方法:采用反相高效液相色谱法,Kromasil ODS C18分析柱(5μm,250 mm×4.6 mm),流动相:甲醇-乙腈-甲酸-水(30∶10∶1∶59),检测波长286 nm,流速1.0 mL/min,柱温25℃。结果:丹酚酸B在0.126 8μg/mL～2.536μg/mL范围内线性关系良好(r=0.999 4),平均回收率为96.5%,RSD=1%(n=5)。结论:该法操作简便、准确,适用于金钱利胆丸的质量控制。Objective：To establish a method for determination of salvianolic acid B content in Jinqian Lidan pill. Methods：HPLC was performed on Kromasil ODS C18 column （5 μm, 250 mm×4.6 mm）with methanol-acetonitrile- formic acid -water （30：10：1：59）as mobile phase. Detection wavelength was set at 286 nm. Flow rate was at 1.0 mL/min and column temperature was at 25 ℃. Result：The linear range of salvianolic acid B was within the range of 0.126 8μg/mL 2.536 μg/mL （r=0.999 4）.The average recovery rate was 96.5%,and RSD was 1%（n=5）. Conclusion：The method is simple and accurate, and suitable for quality control of Jinqian Lidan pill.

关 键 词：高效液相色谱法 金钱利胆丸 丹酚酸B 

分 类 号：R285.5[医药卫生—中药学]