盐生植物盐地碱蓬质膜Na^+/H^+逆向转运蛋白基因片段的克隆及其序列分析  

Cloning and sequence analysis of a plasma membrane Na^+/H^+ antiporter fragment from halophyte Suaeda salsa

作　　者：王生银[1] 马清[1] 王锁民[1] 

机构地区：[1]草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2012年第6期918-923,共6页Pratacultural Science

基　　金：国家自然科学基金(31072073);教育部博士学科点专项科研基金(20090211110001)

摘　　要：为研究盐地碱蓬(Suaeda salsa)Na+长距离运输的分子机制,根据其他植物质膜Na+/H+逆向转运蛋白SOS1基因的保守序列设计一对简并引物,以盐地碱蓬根系总RNA为模板,采用RT-PCR方法克隆出SOS1基因片段,以期为盐地碱蓬SOS1全长基因的克隆、表达调控等研究奠定基础。序列分析结果表明,该基因片段长度为736bp,编码244个氨基酸;该序列与其他高等植物SOS1核苷酸序列的同源性在74%以上,氨基酸序列同源性则在61%以上。To investigate long-distance transport of Na^＋ in Suaeda salsa, degenerate primers were designed based on the conserved sequences of the SOS1 genes from other plants. Total RNA was extracted from the roots of S. salsa and the SOS1 gene fragment was obtained by reverse transcription polymerase chain reac-tion （RT-PCR） aiming to provide the foundation for cloning full-length SOS1 gene and expression regula-tion. The sequencing result showed that the SOS1 gene fragment （736 bp） from S. salsa encoded 224 ami-no acids. Homology comparison showed that the SOS1 gene fragment shared over 740% nucleotide and 61% amino acid similarity to SOSI fragments from other plants.

关 键 词：盐地碱蓬 SOS1基因 克隆 序列分析 

分 类 号：S54[农业科学—作物学]