香菇酶解物对小鼠免疫及肠道菌群的影响研究  

The effect of enzymatic hydrolysate of Lentinula edodes on the immunity and intestinal flora in mice

作　　者：王春晖 陈海强 胡汝晓 黄晓辉 彭运祥 谭周进[2] 

机构地区：[1]湖南省食用菌研究所,湖南长沙410013 [2]湖南中医药大学医学院,湖南长沙410208

出　　处：《微生物学通报》2012年第6期820-826,共7页Microbiology China

基　　金："十一五"国家科技支撑计划课题(No.2008BADAIB07)

摘　　要：【目的】研究香菇酶解物对小鼠免疫及肠道菌群的影响。【方法】首先采用环磷酰胺(CY)造模法制作小鼠免疫抑制模型,然后对其中两组分别灌胃香菇未酶解物和香菇酶解物,最后观察疗效、测定免疫指标和试验小鼠肠道中细菌总数、大肠杆菌数、乳酸菌数和真菌总数等。【结果】香菇酶解物可使受免疫抑制的小鼠(活跃度、粪便、毛皮、体重)恢复到正常水平,促进免疫器官脾脏及胸腺的发育(P<0.05),增强腹腔巨噬细胞的吞噬活性(P<0.01)。同时提高小鼠肠道中以乳酸菌为主的有益菌数量(P<0.01),抑制大肠杆菌等致病菌的生长(P<0.01)。【结论】香菇酶解物可以提高小鼠的免疫功能,改善肠道菌群环境,对肠道功能有一定的调节作用,有较高的食用和药用价值。[Objective] To research the effect of enzymatic hydrolysate of Lentinula edodes onimmune function and intestinal flora in mice. [Methods] Firstly, the immunosuppression model was produced by cyclophosphamide （CY） modeling method. And then one group was filled with no enzymatic hydrolysate by stomach and another group with enzymatic hydrolys- ate. Lastly, curative effect, immune index, the total number of intestinal bacteria, E. coli, Lactobacillus and Fungi in mice were determined. [Results] Enzymatic hydrolysate of Len- tinula edodes could make immune suppression of mice, such as active degree, feces, fur and weight, return to normal levels. It also could improve the spleen and thymus index （P〈 0.05）, promote phagocytic activity of macrophages （P〈0.01）. Meanwhile, it could improve the number of Lactobacillus which is the main beneficial bacteria in mice intestine （P〈0.01） . And it could restrain the growth of pathogenic bacteria such as E. coli （P〈0.01）. [Conclu- sion] The enzymatic hydrolysate of Lentinula edodes could improve the immune function and the intestinal microflora environment. It could adjust intestinal function of mice. The enzy- matic hydrolysate ofLentinula edodes had high edible and medicinal value.

关 键 词：香菇酶解物 小鼠 免疫功能 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]