5-羟色胺1受体对酸灌注脊髓损伤猫膀胱排尿的影响  

Effect of 5-serotonin 1 receptor agonists on acetic acid stimulated micturition in chronic spinal cord injury cats

作　　者：吴刚[1] 程曙杰[2] 曹海兵[3] 陈加生[1] 俞建军[1] 谷宝军[1] 

机构地区：[1]上海交通大学附属第六人民医院泌尿外科,200233 [2]上海市长宁区中心医院 [3]苏州大学

出　　处：《中华实验外科杂志》2012年第6期1022-1024,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30872568）;上海市科委启明星追踪资助项目（09QH1402000）

摘　　要：目的观察5-羟色胺1（5-HT1）受体激动剂对酸灌注脊髓损伤（SCI）猫膀胱排尿的影响。方法手术彻底离断雌猫脊髓12只，术后饲养2个月，氯醛糖麻醉猫后予膀胱置管，注入0．5％乙酸溶液同时行膀胱压力测定。静脉给与5-HT1A受体激动剂8-OH—DPAT（0．30～30．00ug/kg）或GR-46611（0．03～300．00ug/kg），最后给予5-HT1A受体拮抗剂WAY-100635（300．00ug／kg），记录膀胱容量阈值、膀胱容量、残尿量、排尿量和血压，同时记录尿道外括约肌肌电图（EUS—EMG）。结果8-OH-DPAT作用酸灌注脊髓损伤猫后，其膀胱容量阈值、膀胱容量、残尿量等均呈剂量依赖性增加，剂量≥10ug／k时反应明显，差异有统计学意义。而GR-46611无类似效应。WAY-100635能逆转8-OH—DPAT的大部分效应，但对GR-46611无影响。8-OH—DPAT和GR-46611对EUS—EMG均无作用。结论5-HT1a受体激动剂可增加生理盐水灌注及酸灌注脊髓损伤猫的膀胱容量，提示以后可能作为临床上治疗脊髓损伤患者的药物，改善慢性脊髓损伤患者的膀胱过度活动及增加脊髓损伤患者的膀胱容量。Objective To evaluate the effects of 5-serotonin 1 （5-HT1 ） receptor agonists on acetic acid stimulated micturition in cats with chronic spinal cord injury （ SCI ）. Methods Chloralose-anes- thetized SCI cats were catheterized through the bladder dome for filling cystometry during 0. 5% acetic acid infusion. Dose-response curves for （R）-8-OH-DPAT （0. 30-30. 00 ug/kg, i. v. ） or GR 46611 （0. 03- 300. 00 ug/kg, i.v. ） were followed by 5-HT1A antagonist WAY-100635 （300. 00 ug/kg）. Threshold volume, bladder capacity, residual volume, micturition volume, and arterial pressure were measured and the external urethral sphincter electromyogram （EUS-EMG） was recorded. Results Acid-infused SCI cats responded to （R）-8-OH-DPAT but not GR-46611 with dose-dependent increases in threshold volume, capacity, and residual volume, significantly at the dose above 10 ug/kg. Effects of （R）-8-OH-DPAT were largely reversed by WAY 100635. Neither （R）-8-OH-DPAT nor GR-46611 augmented EUS-EMG activity. Conclusion Because 5-HT1A receptor agonists increase bladder capacity under either saline or acid in- fused conditions, they are promising candidates for reducing bladder hyperactivity and increasing bladder capacity in patients with chronic SCI.

关 键 词：脊髓损伤 排尿 5-羟色胺受体 猫 

分 类 号：R651.2[医药卫生—外科学]