烟碱对内脏动脉阻断性休克大鼠的保护效应  

Beneficial roles of nicotine on rats with splanchnic artery occlusion shock

作　　者：梁辉[1] 吴远波[1] 薛娜[1] 李建国[1] 周青[1] 

机构地区：[1]武汉大学中南医院重症医学科,430071

出　　处：《中华实验外科杂志》2012年第6期1080-1082,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30972852）

摘　　要：目的观察特异性N型胆碱能激动剂烟碱对内脏动脉阻断性（SAO）休克大鼠的保护效应。方法采用阻断肠系膜上动脉和腹腔干动脉45min，然后开放夹闭动脉恢复再灌注的方法复制SAO休克模型。将40只健康成年雄性SD大鼠随机分为5组：假手术组、休克组、烟碱组、六烃季胺组和a银环蛇毒素（a—BGT）组。恢复再灌注60min，经左颈总动脉采血用于血浆丙二醛（MDA）含量测定，并无菌留取距回盲瓣10cm处回肠组织，用于其髓过氧化物酶（MPO）、一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）活性测定和病理学检查。结果与休克组大鼠比较，烟碱组大鼠血浆MDA含量、回肠组织MPO和NO的含量明显减少（P〈0．05），iNOS免疫组织化学吸光度值明显降低（P〈0．01），回肠组织的病理学损伤也明显减轻。经六烃季胺或a—BGT拮抗后，烟碱处理的上述保护效应则被抵消。六烃季胺和a-BGT组比较，大鼠血浆MDA及回肠组织MPO、NO含量和iNOS的表达差异均无统计学意义（P〉0．05）。结论烟碱具有减轻SAO休克所致大鼠回肠组织损伤的效应。其作用机制可能与激活胆碱能抗炎通路有关。Objective To investigate the beneficial roles of nicotine, a selective cholinergic agonist, on rats with splanchinc artery occlusion （SAO） shock. Methods Forty male anaesthetized Sprague- Dawley rats, which were randomly divided into 5 groups, were subjected to clamping of the splanchnic arteries for 45 min, followed by release of the clamp to recover reperfusion. This surgical procedure resulted in an irreversible state of shock （SAO shock）. Sham operated animals were used as controls. All the animals were implanted with catheter into left common artery to connect pressure transducer for continuously monitoring mean aortic pressure （MAP）. At 60 rain after reperfusion, all animals were sacrificed for biochemical studies and histological examination. Serum level of malondialdehyde （MDA） and content of myeloperoxidase （MPO） and nitric oxide （ NO）, expression of inducible nitric oxide synthase （iNOS） in ileum, and pathologic changes of ileum were examined. Results Compared with SAO shock group, the serum level of MDA, the tissue levels of MPO, NO and iNOS in the ileum were significantly decreased （P 〈 0. 05 or P 〈 0. 01） and the ileum injury was attenuated in the nicotine group. Administration of specific nAch receptor antagonist worsened SAO shock-induced tissue injury, and there was no difference in con- centration of serum MDA, and in contents of MPO, NO, and iNOS of ileum between two antagonist groups （P 〉 0. 05 ）. Conclusion The results suggest that nicotine may play beneficial roles on SAO shock rats through activation of cholinergic anti-inflammatory pathway.

关 键 词：休克 缺血 再灌注损伤 烟碱 回肠 

分 类 号：R459.7[医药卫生—急诊医学]