药对蛇床子—补骨脂对乳腺癌骨转移裸鼠生存时间与骨损伤的影响  

Influences of coupled medicinal-Shechuangzi(Fructus Cnidii) and Buguzhi(Fructus Psoraleae) on survival time and bone injury in nude mice with breast cancer with bone metastasis

作　　者：程旭锋[1,2] 刘琦[3] 刘胜[2] 张新峰[4] 杨顺芳[5] 

机构地区：[1]河南中医学院第一附属医院乳腺外科,河南450000 [2]上海中医药大学附属龙华医院乳腺外科 [3]河南中医学院科研处 [4]河南省肿瘤医院中西医结合科 [5]上海交通大学附属胸科医院实验中心

出　　处：《北京中医药大学学报》2012年第5期317-322,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.30772811);河南省教育厅自然科学基金项目(No.2010B360005);郑州市科技局科技攻关计划(No.112PPTSF319-14);郑州市金水区技术研究与开发经费支持项目(No.20093542)

摘　　要：目的研究药对蛇床子—补骨脂对裸鼠乳腺癌骨转移生存时间、破骨细胞活性及骨损伤的影响,探讨该药对治疗乳腺癌骨转移的最佳配伍关系。方法用左心室注射MDA-MB-231BO细胞法对裸鼠进行造模,观察蛇床子—补骨脂药对(2.56 g/只)的作用,以唑来膦酸(0.2 mg/kg)作为阳性对照药。将84只裸鼠模型随机分为A、B组,每组42只,每组再随机分为模型组、唑来膦酸组和蛇床子—补骨脂4∶0、0∶4、1∶3、2∶2、3∶1组,每组6只,实验B组另配正常组裸鼠6只。实验A组观察不同配比药对干预后各组模型的生存时间;实验B组不同配比药对干预6周后取材,TRACP染色观察各组破骨细胞的形态和数量,检测骨转移灶M-CSF、PTHrP的基因与蛋白的表达。结果各干预组均能显著延长裸鼠模型的生存时间(P<0.05,P<0.01),中药组以2∶2配伍最好,疗效接近唑来膦酸组;各干预组均可在一定程度上抑制乳腺癌骨转移裸鼠骨转移组织中破骨细胞的活性(P<0.05,P<0.01),中药组以2∶2配伍最好,疗效接近唑来膦酸组;各干预组均可不同程度地下调M-CSF、PTHrP的基因与蛋白的表达(P<0.05,P<0.01),中药组以2∶2配伍最好。结论基于温肾法的蛇床子—补骨脂药对可以抑制乳腺癌骨转移,该药对最佳疗效配伍比例可能是2∶2。Objective To study the influences of coupled Buguzhi （Fructus Psoraleae） on survival time, activity of breast cancer with bone metastasis, and discuss the best treatment of breast cancer with bone metastasis. Methods medicinal-Shechuangzi （Fructus Cnidii） and osteoclast and bone combination of the The model of nude injury in nude mice with coupled medicinal in the mouse was established by using left ventricle injection of MDA-MB-231BO cells. The effect of coupled medicinal-Shechuangzi and Buguzhi（2.56 g/mouse） was observed taking zoledronic acid as a positive control medicinal. All nude mice （n =84） were randomly divided into（0.2 mg/kg） group A and group B （each n =42）, and each group were randomly divided again into model group, zoledronic acid group, 4 parts Shechuangzi to 0 part Buguzhi group （4 to 0 group）, 0 part Shechuangzi to 4 parts Buguzhi group （0 to 4 group）, 1 part .Shechuangzi to 3 parts Buguzhi group （ 1 to 3 group）, 2 parts Shechuangzi to 2 parts Buguzhi group （2 to 2 group）, 3 parts Shechuangzi to 1 parts Buguzhi group （3 to 1 group, each n = 6）. A normal group （n = 6） was additionally set into group B. The survival time was observed in group A after intervention by different proportions of medicinal. The samples were collected 6 weeks after intervention by different proportions of medicinal in group B. The changes of morphology and quantity of osteoclasts were observed after TRACP staining, and the expressions of bone metastasis focus. Results The survival in nude mouse model （P 〈 O. 05, P 〈 O. O1 ） almost as the same as that in zoledronic acid genes and proteins of M-CSF and PTHrP were detected in time was significantly prolonged in all intervention groups , and the effect was the best in 2 to 2 group, which was group. The activity of osteoclasts was inhibited to some extent in all intervention groups （P 〈 0. 05, P 〈 0.01 ）, and the effect was the best in 2 to 2 group, which was almost as the same as that in zoledronic acid group

关 键 词：乳腺癌 骨转移 药对 蛇床子-补骨脂 小鼠 

分 类 号：R285.5[医药卫生—中药学]