半夏悬浮培养细胞的EMS诱变和耐高温突变体筛选  

EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata

作　　者：陶兴魁[1,2] 张爱民[1] 薛建平[1] 滕井通[1] 卢河东[1] 薛涛[1] 

机构地区：[1]淮北师范大学生命科学学院资源植物生物学安徽省重点实验室,安徽淮北235000 [2]宿州学院化学与生命科学学院,安徽宿州234000

出　　处：《中国中药杂志》2012年第12期1731-1733,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973963);安徽省自然科学基金项目(090413252);安徽高校省级自然科学研究重点项目(KJ2009A160);宿州学院特色种植业苗种生产工程技术研究中心项目(2011YKF18)

摘　　要：目的:确定EMS诱变处理半夏悬浮细胞的最适浓度和处理时间。方法:用0.1%,0.2%,0.4%,0.6%的EMS溶液,处理时间为0.5,1.0,2.0 h,对半夏悬浮细胞系进行诱变处理。结果与结论:结果表明,不同浓度的EMS和不同时间处理半夏悬浮细胞,结果有明显差异,在每个处理时间下,均是随着EMS浓度的增加而悬浮细胞的存活率逐渐下降。EMS诱变半夏悬浮细胞的适宜剂量为0.4%,最佳处理时间为1 h。Objective： To determine the optimal concentration and processing time of EMS mutation for suspension ceils from Pinellia ternata. Method： Under four EMS concentration gradients （0.1% , 0.2% , 0.4% ,0.6% ） and three processing time gradi- ents （0.5,1.0,2.0 h）, the suspension cells of P. ternata were mutagenized. Result and Conclusion： The results showed that the survival rate was significantly different under the different concentrations of EMS and the different processing time. In the same process- ing time, the EMS concentrations were increased, but the suspension cells survival rate decreased gradually. The optimum EMS con- centration for the mutagenesis was 0.4% and the best processing time was 1 hour.

关 键 词：半夏 悬浮细胞 EMS诱变 耐高温突变体 

分 类 号：S567.239[农业科学—中草药栽培]