人参Ri质粒转化及代谢产物对HepG2细胞的作用  

Ri plasmid transformation and metabolism of ginseng products on HepG2 cells

作　　者：杨晶[1] 李天航[1] 郭咏昕[1] 官丽莉[1] 李海燕[1] 李校堃[1] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118

出　　处：《中国中药杂志》2012年第12期1738-1742,共5页China Journal of Chinese Materia Medica

基　　金：国家高技术研究发展计划(863)项目(SQ2010AA1000691008)

摘　　要：目的:发状根生产的次生代谢产物含量比植物自身合成的含量高几倍甚至几十倍,利用人参发状根可以生产大量人参皂苷,并有效的抑制癌细胞的增值。方法:通过正交试验设计筛选人参发状根诱导的条件,通过发状根生物积累量和皂苷含量的测定确定培养条件,然后利用MTT法测定了人参皂苷对HepG2细胞的影响。结果:本实验优化了人参发状根的最佳诱导条件,吸光度A 0.6,侵染时间为10 min,预培养时间为3 d,筛选出发状根的最佳培养条件,MS培养基,pH 6.1,24℃培养时,发状根的生物积累量和皂苷含量均较高,采用人参总皂苷对肝癌细胞HepG2的抑制效果进行研究,证实人参总皂苷对HepG2的抑制率与给药浓度呈正相关。结论:筛选出最适的人参发状根诱导及培养条件,不同部位的总皂苷对肝癌细胞HepG2的增殖均具有抑制作用。Objective： To study the Ri plasmid transformstion and metabolism of ginseng products on HepG2 cells. Method： The inhibitory effect of ginseng saponin on hepatoma HepG2 cells was studied. The hairy root-induced conditions were screened by or- thogonal experimental design. The culture conditions were determined through hairy root biomass accumulation and saponin content. The effect of ginsenoside on HepG2 ceils was determined by MTY assay. Result and Conclusion： The optimal ginseng hairy root indu- cing conditions ： A = 0.6, infection time of 10 min, pre-incubation time for the 3 d. The best culture conditions： MS medium, pH 6. 1, 24 ~C. At those conditions the hairy root bio-accumulation and saponin content were higher. The results of ginseng saponins on the inhibitory effect of HepG2 cells showed that inhibition of ginseng saponins on HepG2 was the concentration positively related.

关 键 词：人参发状根 RI质粒 人参皂苷 HEPG2细胞 

分 类 号：R285.5[医药卫生—中药学] R735.7[医药卫生—中医学]