莪术醇对斑马鱼的血管生成活性及作用机制研究  

Effect and mechanism of curcumol on angiogenesis activity of zebrafishes

作　　者：田丽莉[1] 董建勇[1] 黄长江[2] 

机构地区：[1]温州医学院药学院,浙江温州325035 [2]温州医学院水域科学与环境生态研究所,浙江温州325035

出　　处：《中国中药杂志》2012年第12期1822-1825,共4页China Journal of Chinese Materia Medica

基　　金：浙江省中医管理局重大科研项目(2008ZA016)

摘　　要：目的:初步研究莪术醇促进血管生成的活性及作用机制,为临床用药提供依据。方法:采用斑马鱼生物模式,观察莪术醇对斑马鱼胚胎体节间血管生长、成鱼剪尾后血管再生和仔鱼切尾后组织再生情况。采用相对荧光定量PCR法检测仔鱼切尾后体内血管内皮生长因子(VEGFA)及受体(VEGFR2)基因的表达。结果:莪术醇能使斑马鱼胚胎体节间血管侧生出新的血管,使成鱼剪尾后血管再生加快,使仔鱼切尾后尾部再生加快。且莪术醇能提高仔鱼体内VEGFA及VEGFR2基因的表达量,促进仔鱼切尾后的尾部再生。结论:莪术醇能促进斑马鱼的血管生成,提高仔鱼切尾后体内VEGFA及VEGFR2基因的表达量,促进尾部再生。Objective： To conduct a preliminary study on the effect of curcumol in promoting angiogenesis activity and its mechanism in zebrafishes, in order to provide basis for clinical prescription. Method： Zebrafishes biological model was established to observe eurcumol＇s effect on embryo blood vessel growth, blood vessel regeneration of adult fishes after tail-cutting and tissue regenera- tion of fish fries after tail-cutting. The relative fluorescence quantitative PCR method was adopted to determine the gene expression of vascular endothelial growth factor （VEGFA） and receptor VEGFR2 of fish fries after tail-cutting. Result： Curcumol contributed to an- giogenesis of intersegmental blood vessels in zebrafishes embryos and speed up regeneration of blood vessels in adult fishes after tail- cutting. Furthermore, cureumol can increase the gene expression of VEGFA and VEGFR2 in fish fries. Conclusion： Curcumol can promote angiogenesis in zebrafishes, and enhance the gene expression of VEGFA and VEGFR2 in fish fries after tail-cutting and speed up the regeneration of their tails.

关 键 词：斑马鱼 莪术醇 血管生成 VEGFA VEGFR2 

分 类 号：R285.5[医药卫生—中药学]