基于UPLC-Q-TOF-MS及数据自动处理技术的刺五加提取物中异嗪皮啶及其代谢产物分析  

Analysis on isofraxidin contained in Acanthopanax senticosus extracts and its metabolites by UPLC-Q-TOF-MS and automatic data processing technique

作　　者：刘树民[1] 孙强[1] 白云[2] 卢芳[1] 闫广利[1] 包顺茹[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040 [2]黑龙江中医药大学基础医学院,黑龙江哈尔滨150040

出　　处：《中国中药杂志》2012年第12期1840-1844,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(30901974);国家“重大新药创制”科技重大专项(2009ZX09103-329);教育部“春晖计划”合作科研项目(Y-30);教育部“春晖计划”项目(Z2009-1-15032)

摘　　要：目的:分析大鼠灌胃刺五加提取物后血浆、胆汁、尿液和粪便中异嗪皮啶(M0)及其代谢产物。方法:健康雄性wistar大鼠按325 mg.kg-1的剂量灌胃给予刺五加提取物,分别采集给药后1,1.5,2,4,6 h肝门静脉血液并用SPE技术制备血浆;采集0～12 h胆汁并用SPE技术制备;0～12 h,12～24 h分别采集尿液和粪便样品并制备样品,采用UPLC-Q-TOF-MS技术和Metabolynx XS软件联合的方法分析。结果:在大鼠血浆、胆汁、尿液和粪便中检测到M0,在血浆和胆汁中检测到代谢物异嗪皮啶葡萄糖醛酸苷(M1)。其中M1是首次报道的异嗪皮啶代谢产物。结论:异嗪皮啶在大鼠体内以葡萄糖醛酸形式代谢,最终又以异嗪皮啶形式排出体外。Objective： To analyze isofraxidin and its metabolites in rat plasma, bile, urine and teces after the oral administra- tion of A. senticosus extracts. Method： After the oral administration of 325 mg ·kg^-1 of A. senticosus extracts, rat blood samples were collected at 1, 1.5, 2, 4, 6 h and bile samples were collected during 0-12 h. The above samples were prepared by SPE. Their urine and feces samples were collected during 0-12 h and 12-24 h. These samples were analyzed by UPLC-Q-TOF-MS and processed using Metabolynx XS. Result： M0 was detected in rat plasma, bile, urine and feces, isofraxidin glucuronide （MI ） was detected in rat plas- ma and bile, which was first reported as the metabolite of isofraxidin. Conclusion ： Isofraxidin can be metabolized in the form of glucu- ronidation in vivo and evacuated in the form of isofraxidiu.

关 键 词：刺五加提取物 异嗪皮啶 代谢产物 UPLC—Q—TOF—MS 

分 类 号：R285.5[医药卫生—中药学]