柴胡皂苷对芍药苷在Caco-2细胞模型中吸收转运的影响  

Impact of saikosaponin on absorption and transport of paeoniflorin in Caco-2 cell model

作　　者：陈彦[1] 王晋艳[1] 辛然[1] 谭晓斌[1] 

机构地区：[1]国家中医药管理局中药释药系统重点研究室,江苏省中医药研究院中药新型给药系统重点实验室,江苏南京210028

出　　处：《中国中药杂志》2012年第12期1850-1854,共5页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局中医药科学技术研究专项(06-07ZP18);江苏省中医药领军人才项目(2009)

摘　　要：目的:研究柴胡皂苷a,d对芍药苷在Caco-2细胞模型中转运的影响。方法:用超高压液相法测定药物浓度,计算表观渗透系数,比较不同浓度芍药苷溶液、及芍药苷配伍柴胡皂苷a,d后在Caco-2细胞模型中双向转运的差别;电阻仪测定电阻,比较试验前后细胞电阻的差异。结果:芍药苷通过Caco-2细胞单层的转运量4 h内随时间延长呈线性增大,其吸收渗透系数较小,浓度为20,50,100μmol.L-1时,吸收渗透系数分别为(0.98±0.10)×10-6,(0.92±0.09)×10-6,(0.89±0.04)×10-6cm.s-1;芍药苷与柴胡皂苷a,d配伍后,吸收渗透系数与单体比较分别增加了2.37,2.54倍,且试验后细胞电阻有显著降低。结论:柴胡皂苷a,d能促进芍药苷的肠道吸收,可能与柴胡皂苷能打开Caco-2细胞间的紧密连接有关。Objective： To study the impact of saikosaponin on absorption and transport of paeoniflorin in Caco-2 celt model. Method： The comcentration of paeoniflorin in cell culture medium was measured by UPLC and the apparent permeability coefficients （Ppp） was calculated to study differences in hi-direction transport of paeoniflorin solutions of different concentrations and its compati- bility with saikosaponin a, and saikosaponin d in Caeo-2 cell model. Meanwhile, the electric resistance of Caco-2 cell was determined before and after the experiment. Result ： The amount of paeoniflorin increased linearly with the transport of Caco-2 cell monolayer in 4 h, with a lower absorptive permeability, which was （0.98±0.10）×10^6,（0.92±0.09）×10^6,（0.89±0.04）×10^6cm·s^-1 at the concentration of 20, 50, 100 μmol·L^-1 , respectively. After compatibility with saikosaponin a and saikosaponin d, the absorptive permeability of paeoniflorin increased by 2. 37 times and 2. 54 times, respectively, while the electric resistance of Caeo-2 cell was de- creased significantly after the experiment. Conclusion： Saikosaponin a, d can enhance the absorption of paeoniflorin in Caco-2 cell monolayer, which may be related to saikosaponin＇s ability to open up intercellular tight junctions among Caco-2 cells.

关 键 词：芍药苷 柴胡皂苷A 柴胡皂苷D CACO-2细胞单层模型 

分 类 号：R285.5[医药卫生—中药学]